Individualized bacterial treatment of pancreatic cancer
An individualized bacterial treatment of cancer is provided. The treatment includes a strain of bacteria modified by in-vivo passage through tumor grafts in experimental animals, where the modified strain exhibits enhanced cancer cell-targeting of a specific malignancy arising in a unique individual to the corresponding parent strain of bacteria. The treatment uses this modified strain for the treatment human solid-tumor malignancies by inoculating an individual with a quantity of the strain; and repeating inoculations at periodic intervals where repeated inoculations tend to progressively eliminate the solid tumor malignancy in the individual.
1. A method for creating a strain of enhanced cancer cell-targeting bacteria for individualized cancer therapy, said method comprising:
(a) harvesting cancer cells from an individual;
(b) transplanting a quantity of said cancer cells into a quantity of experimental animals;
(c) incubating the experimental animals to establish a cancer tissue graft within the experimental animals;
(d) inoculating a Salmonella typhimurium A1 into a said experimental animal containing established said cancer tissue graft;
(e) removing said cancer tissue graft from said experimental animal inoculated with said Salmonella typhimurium A1 following a period of incubation of the inoculated said experimental animal;
(f) extracting a Salmonella typhimurium A1-R from said cancer tissue graft removed from the experimental animal inoculated with said Salmonella typhimurium A1-R ;
(g) incubating said Salmonella typhimurium A1-R under conditions suitable for bacterial multiplication;
(h) selecting a said Salmonella typhimurium A1-R ;
(i) incubating the selected said Salmonella typhimurium A1-R under conditions suitable for bacterial multiplication;
(j) inoculating a quantity of selected said Salmonella typhimurium A1-R into a second said experimental animal containing established said cancer tissue graft;
(k) removing said cancer tissue graft from the said experimental animal inoculated with a quantity Salmonella typhimurium A1-R following a period of incubation;
(l) extracting Salmonella typhimurium A1-R from said cancer tissue graft removed from the experimental animal;
(m) incubating said bacteria R1 under conditions suitable for bacterial multiplication; and
(n) selecting a Salmonella typhimurium R1 .
2. The method of claim 1 wherein said enhanced cancer cell-targeting bacteria is created by at least one passage through cancer cells in vitro.
3. The method of claim 1 wherein the Salmonella typhimurium A1 is auxotrophic for one or more essential nutrients.
4. The method of claim 1 wherein the Salmonella typhimurium A1 is auxotrophic for arginine and leucine.
5. The method of claim 1 further comprising creating additional strains of enhanced cancer cell-targeting Salmonella typhimurium A1 in response to repeating a portion of the method n additional times sequentially with strains of said Salmonella typhimurium A-Rn .
6. The method of claim 1 further comprising labeling the Salmonella typhimurium A1 with a fluorescent label.
7. The method of claim 1 further comprising labeling the Salmonella typhimurium A1 with a fluorophore.
8. The method of claim 1 further comprising labeling the Salmonella typhimurium A1 with a fluorescent protein.
9. The method of claim 1 further comprising labeling the Salmonella typhimurium A1 with a fluorescent antibody.
10. The method of claim 1 further comprising labeling the nuclei of the harvested cancer cells with a fluorescent label.
11. The method of claim 1 further comprising labeling the nuclei of the harvested cancer cells with a fluorescent protein; and a fluorescent antibody.
12. The method of claim 1 further comprising labeling cytoplasm of the harvested cancer cells with a fluorescent label.
13. The method of claim 1 further comprising labeling cytoplasm of the harvested cancer cells with a fluorophore.
14. The method of claim 1 further comprising labeling cytoplasm of the harvested cancer cells with a fluorescent protein.
15. The method of claim 1 further comprising labeling cytoplasm of the harvested cancer cells with a fluorescent antibody.
16. The method of claim 1 further comprising labeling the nucleus and the cytoplasm of the harvested cancer cells with fluorescent labels.
17. The method of claim 1 , wherein said cancer cells are a pancreatic cancer.
18. The method of claim 1 , wherein said cancer cells are a sarcoma.
19. The method of claim 1 , wherein said cancer cells are a lung cancer.
20. The method of claim 1 , wherein said cancer cells are a breast cancer.
21. The method of claim 1 , wherein said cancer cells are a colon cancer.