Cotton event pDAB4468.18.07.1 detection method
Cotton event pDAB4468.18.07.1 comprises gene expression cassettes which contain genes encoding aad-12 and pat, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection. Embodiments of the subject invention provide polynucleotide-related event detection methods.
1. A method of detecting cotton event pDAB4468.18.07.1 in a sample comprising cotton DNA, said method comprising:
contacting said sample with a first primer at least 10 bp in length that selectively binds to a flanking sequence within by 1-2886 of SEQ ID NO:1 or the complement thereof, and a second primer at least 10 bp in length that selectively binds to an insert sequence within by 2887-3206 of SEQ ID NO:1 or the complement thereof; and
assaying for an amplicon generated between said primers, wherein the amplicon consists of a polynucleotide comprised within SEQ ID NO:1.
2. The method according to claim 1 , wherein said sample comprising cotton DNA is obtained from cotton plants comprising event pDAB4468.18.07.1 that have been deposited under ATCC accession number PTA-12456.
3. The method according to claim 1 , wherein the first primer comprises the polynucleotide of SEQ ID NO:5, and wherein the second primer comprises the polynucleotide of SEQ ID NO:6.
4. The method of claim 3 , wherein said sample comprising cotton DNA is obtained from cotton plants comprising event pDAB4468.18.07.1 that have been deposited under ATCC accession number PTA-12456.