Methods of identifying a subject as having heart failure
Measurement of circulating ST2 and/or IL-33 concentrations is useful for the prognostic evaluation of subjects, in particular for the prediction of adverse clinical outcomes, e.g., mortality, and the detection of severe disease.
1. A method of identifying, a subject having undiagnosed chest pain or undiagnosed chest discomfort as having heart failure, the method comprising:
(a) selecting a subject having undiagnosed chest pain or undiagnosed chest discomfort;
(b) performing an immunoassay to determine a level of soluble ST2 in a biological sample from the selected subject;
(c) performing an immunoassay to determine a level of one or more adjunct biomarkers selected from the group consisting of: N-terminal (NT)-pro-brain natriuretic peptide (BNP), BNP, NT-pro-atrial natriuretic peptide (ANP), proANP, and ANP in a biological sample from the selected subject;
(d) comparing the level of soluble ST2 in the selected subject to a reference level of soluble ST2;
(e) comparing the level of the one or more adjunct biomarkers in the selected subject to a reference level of the one or more adjunct biomarkers; and
(f) identifying a selected subject having an elevation in the level of soluble ST2 compared to the reference level of soluble ST2 and an elevation in the level of the one or more adjunct biomarkers compared to the reference level of the one or more adjunct biomarkers as having heart failure by performing echocardiography.
2. The method of claim 1 , wherein the reference level of soluble ST2 is a level of soluble ST2 in a subject that has no disease or has no acute disease.
3. The method of claim 1 , wherein the reference level of soluble ST2 is a threshold level of soluble ST2.
4. The method of claim 1 , wherein the subject in step (a) is previously undiagnosed.
5. The method of claim 1 , wherein the biological sample in step (b) and step (c) comprises serum or plasma.
6. A method for identifying a subject having undiagnosed chest pain or undiagnosed chest discomfort as having heart failure, the method comprising:
(a) selecting a subject having undiagnosed chest pain or undiagnosed chest discomfort;
(b) performing an immunoassay to determine a level of soluble ST2 in a biological sample from the selected subject;
(c) performing an immunoassay to determine a level of one or more adjunct biomarkers selected from the group consisting of: N-terminal (NT)-pro-brain natriuretic peptide (BNP), proBNP, BNP, NT-pro-atrial natriuretic peptide (ANT), proANP, and ANP in a biological sample from the selected subject;
(d) comparing the level of soluble ST2 in the selected subject to a reference level of soluble ST2;
(e) comparing the level of the one or more adjunct biomarkers in the selected subject to a reference level of the one or more adjunct biomarkers; and
(f) identifying a selected subject having an elevation in the level of soluble ST2 compared to the reference level of soluble ST2 and an elevation in the level of the one or more adjunct biomarkers compared to the reference level of the one or more adjunct biomarkers as having heart failure by performing cardiac catheterization.
7. The method of claim 6 , wherein the reference level of soluble ST2 is a level of soluble ST2 in a subject that has no disease or has no acute disease.
8. The method of claim 6 , wherein the reference level of soluble ST2 is a threshold level of soluble ST2.
9. The method of claim 6 , wherein the subject is previously undiagnosed.
10. The method of claim 1 , wherein the method is performed in an outpatient clinic.
11. The method of claim 6 , wherein the method is performed in an outpatient clinic.
12. The method of claim 1 , wherein the immunoassay to determine the level of soluble ST2 is an enzyme-linked immunosorbant assay (ELISA).
13. The method of claim 11 , wherein the ELISA is a sandwich ELISA.
14. The method of claim 1 , wherein the immunoassay to determine the level of soluble ST2 utilizes a monoclonal antibody.
15. The method of claim 6 , wherein the immunoassay to determine the level of soluble ST2 is an enzyme-linked immunosorbant assay (ELISA).
16. The method of claim 15 , wherein the ELISA is a sandwich ELISA.
17. The method of claim 6 , wherein the immunoassay to determine the level of soluble ST2 utilizes a monoclonal antibody.
18. The method of claim 14 , wherein the immunoassay to determine the level of soluble ST2 utilizes two different monoclonal antibodies that specifically bind to soluble ST2.
19. The method of claim 17 , wherein the immunoassay to determine the level of soluble ST2 utilizes two monoclonal antibodies that specifically bind to soluble ST2.
20. The method of claim 6 , wherein the biological sample in step (b) and step (c) comprises serum or plasma.
21. The method of claim 1 , wherein the subject has undiagnosed chest pain.
22. The method of claim 1 , wherein the subject has undiagnosed chest discomfort.
23. The method of claim 6 , wherein the subject has undiagnosed chest pain.
24. The method of claim 6 , wherein the subject has undiagnosed chest discomfort.