Patent
The invention provides compositions and methods relating to the elevation of glucoraphanin compared to standard Brassica oleracea varieties. The invention also relates to the production of hybrid varieties having desired glucosinolate contents. The invention further provides plants, plant parts, and seeds comprising such traits and comprising a Myb28 allele from Brassica villosa that is not genetically linked to an ELONG allele from Brassica villosa.
1. A Brassica oleracea plant comprising a chromosomal segment that comprises a Myb28 allele from Brassica villosa and lacking an ELONG allele from Brassica villosa genetically linked to said Myb28 allele, wherein the segment confers elevated glucosinolates relative to a Brassica oleracea plant lacking the Myb28 allele, wherein a sample of seed comprising the chromosomal segment was deposited under ATCC Accession Number PTA-13165.
2. The plant of claim 1 , wherein the plant is a broccoli plant.
3. The plant of claim 1 , wherein the plant is inbred.
4. The plant of claim 1 , wherein the plant is hybrid.
5. The plant of claim 1 , wherein the plant is homozygous for said Myb28 allele from Brassica villosa.
6. The plant of claim 1 , wherein the plant is heterozygous for said Myb28 allele from Brassica villosa.
7. The plant of claim 1 , further comprising an ELONG allele from Brassica oleracea.
8. A seed that produces the plant of claim 1 .
9. A plant part of the plant of claim 1 , wherein the plant part comprises the Myb28 allele but not the ELONG allele from Brassica villlosa.
10. The plant part of claim 9 , wherein the part is a leaf, an ovule, a floret, pollen, a head, or a cell, and wherein the part comprises the Myb28 allele but not the ELONG allele from Brassica villlosa.
11. A progeny of the plant of claim 1 , wherein said progeny comprises said Myb28 allele but not the ELONG allele from Brassica villosa.
12. A part of the progeny of claim 11 selected from the group consisting of a cell, a seed, a root, a stem, a leaf, a head, a flower, and pollen, wherein said part comprises said Myb28 allele but not the ELONG allele from Brassica villosa.