Photodynamic diagnosis, formulations usable as photosensitizers for this purpose, method for the production and use thereof
In the early detection of cancer, in particular bladder cancer, a photodynamic diagnosis uses, as a photosensitizer, a formulation that contains sodium hypericinate that is bonded to polyvinylpyrrolidone or complexed with polyvinylpyrrolidone.
1. A method for photodynamic diagnosis of tumors, comprising:
photosensitizing with a photosensitizer that is a stable complex or a stable compound of a sodium or potassium salt of hypericin and a polymeric complexing agent selected from the group consisting of polyethylene glycol and poly-N-vinyl amide, the photosensitizer containing 0.225 mg of hypericin
wherein the photosensitizer is obtained from a lyophilizate obtained from a solution of the sodium or potassium metal salt of hypericin, the polymeric complexing agent and a buffer system comprising a phosphate buffer or a citric acid buffer.
2. The method according to claim 1 , wherein a photodynamic diagnosis for early detection of cancer is made.
3. The method according to claim 1 , wherein the poly-N-vinyl amide is a polyvinylpyrrolidone (PVP) of various degrees of polymerization and cross-linking.
4. The method according to claim 3 , wherein the polyvinylpyrrolidone is PVP k17, PVP k25 or PVP k30.
5. The method according to claim 1 , wherein a photodynamic diagnosis for detection of bladder cancer, is made.
6. The method according to claim 1 , further comprising the following successive steps:
a) preparing the solution of the sodium or potassium salt of hypericin;
b) dissolving the complexing agent in the solution;
c) adding sufficient of the buffer system to obtain a concentration of 0.0225 mg hypericin/g solution; and
d) lyophilizing the solution to obtain the lyophilizate to obtain the photosensitizer containing the 0.225 mg hypericin.