Simplified basic media for human pluripotent cell culture
Fully defined media that support pluripotent cell viability, proliferation, cloning, and derivation, as well as methods and compositions including these media are described. Methods for deriving iPS cells from adult individuals under defined, xeno-free conditions are also described.
1. A method for deriving an induced pluripotent stem (iPS) cell under defined conditions, the method comprising the step of:
introducing reprogramming factors into a somatic cell in a defined, albumin-free medium that is free of any component obtained from a non-human animal, the medium comprising water, salts, amino acids, vitamins, glucose, an FGF, selenium, transferrin, one of insulin, IGF1, and IGF2, and one of TGF-β and NODAL, each in an amount sufficient to support reprogramming of the somatic cell to derive an iPS cell.
2. The method of claim 1 , wherein the IGF1 is Long-IGF1.
3. The method of claim 1 , wherein the reprogramming step comprises contacting the cell with TGFβ for 5-10 days.
4. The method of claim 1 , further comprising the steps of:
removing the TGFβ after 5-10 days; and
contacting the cells with a medium consisting essentially of:
water, salts, amino acids, vitamins, glucose, an FGF, selenium, one of insulin, IGF1, and IGF2, and transferrin.
5. The method of claim 1 , wherein the reprogramming step comprises contacting the cell with hydrocortisone.
6. The method of claim 1 , wherein the reprogramming step comprises contacting the cell with butyrate.
7. The method of claim 1 , wherein the somatic cell is an adult somatic cell.
8. The method of claim 1 , wherein the reprogramming step comprises introducing a viral vector into the somatic cell.
9. The method of claim 1 , wherein the reprogramming step comprises introducing an episomal vector into the somatic cell.