Fluid control structures in microfluidic devices
Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid. The fluid control structures can be used to implement a variety of sample introduction, preparation, processing, and storage techniques.
1. A method for controlling fluid flow on a microfluidic device, the method comprising:
providing a microfluidic device comprising one or more elastomer membranes sandwiched between a pneumatic layer and a fluidic layer, wherein the fluidic layer comprises a plurality of fluidic channels, each of which comprises of a discontinuity, and a plurality of via holes at the discontinuity, wherein the pneumatic layer comprises a first surface including at least one pneumatic channel facing an elastomer membrane and valve areas aligned with said discontinuities, the fluidic layer comprising a second surface including the plurality of via holes facing the one or elastomer membranes;
controlling fluidic flow across a fluidic channel by varying pneumatic pressure in a pneumatic channel thereby causing an elastomer membrane to deflect at a discontinuity of the fluidic channel to modulate a flow of a fluid volume from one side of the discontinuity, through the plurality of via holes, to the other side of the discontinuity; and
varying pneumatic pressure in one pneumatic channel to thereby cause one or more elastomer membranes to deflect at discontinuities of a plurality of fluidic channels to modulate flow of a plurality of fluid volumes in the plurality of fluidic channels.
2. The method of claim 1 , wherein the microfluidic device further comprises an input valve, an output valve and a diaphragm valve, the input valve, output valve and diaphragm valves being in series and wherein the method further comprises opening the input valve and closing the output valve by varying pneumatic pressure to the one or more of the elastomer membranes, opening the diaphragm valve and closing the input valve by varying pneumatic pressure; and opening the output valve and closing the diaphragm valve, wherein closing the diaphragm valve pumps fluid through the open output valve.
3. The method of claim 1 , wherein causing an elastomer membrane to deflect at a discontinuity of the fluidic channel to modulate a flow of a fluid volume from one side of the discontinuity, through the plurality of via holes, to the other side of the discontinuity comprises causing the elastomer membrane to deflect away from the discontinuity to provide a path for the fluid volume across the discontinuity.
4. The method of claim 1 , wherein causing an elastomer membrane to deflect at a discontinuity of the fluidic channel to modulate a flow of a fluid volume from one side of the discontinuity, through the plurality of via holes, to the other side of the discontinuity comprises causing the elastomer membrane to deflect toward from the discontinuity to prevent flow of the fluid volume across the discontinuity.
5. The method of claim 1 , wherein the fluidic layer is gas impermeable.
6. The method of claim 1 , further comprising varying pneumatic pressure in a plurality of pneumatic channels to thereby cause one or more elastomer membranes to deflect modulate flow of a plurality of fluid volumes in the plurality of fluidic channels.
7. The method of claim 1 , further comprising deflecting one or more of the elastomer membranes within a displacement chamber in the pneumatic layer to thereby form a fluid reservoir in a fluidic channel.
8. The method of claim 1 , wherein varying the pneumatic pressure in the pneumatic channel comprises applying vacuum to the pneumatic channel.
9. The method of claim 1 , wherein varying the pneumatic pressure in the pneumatic channel comprises applying pressure to the pneumatic channel.
10. The method of claim 1 , wherein varying the pneumatic pressure in the pneumatic channel comprises applying pressure or vacuum to a port in the pneumatic layer.
11. The method of claim 1 , wherein the pneumatic layer is plastic.
12. The method claim 1 , wherein the fluidic layer is plastic.
13. The method of claim 1 , wherein the microfluidic device has only one elastomeric layer.