Neurovirulent strain of the West Nile virus and uses thereof
Neuroinvasive and neurovirulent strain of the West Nile virus, named IS-98-ST1, nucleic acid molecules derived from its genome, proteins and peptides encoded by said nucleic acid molecules, and uses thereof.
1. An immunoassay method for detecting a west nile virus (WNV) infection comprising:
(a) providing a biological sample suspected of containing anti-WNV antibodies
(b) contacting the biological sample with an immunoassay composition comprising at least one isolated peptide consisting of an amino acid sequence encoded by a polynucleotide fragment consisting of between 50 and 200 contiguous nucleotides of the coding sequence SEQ ID NO: 1,
wherein the 50 and 200 contiguous nucleotides of the coding sequence SEQ ID NO: 1 include a codon selected from:
the alanine codon at positions 1117-1119 of SEQ ID NO: 1,
the asparagine codon at positions 2518-2520 of SEQ ID NO: 1,
the arginine codon at positions 4018-4020 of SEQ ID NO: 1,
the glycine codon at positions 4462-4464 and/or and the glutamic acid codon at positions 4465-4467 of SEQ ID NO: 1,
the proline codon at positions 6097-6099 and/or the glutamic acid codon at positions 6172-6174 of SEQ ID NO: 1,
the serine codon at positions 7840-7842 of SEQ ID NO: 1,
the asparagine codon at positions 8518-8520 of SEQ ID NO: 1, and
the alanine codon at positions 8794-8796 of SEQ ID NO: 1; and
(c) detecting an immunological reaction between an antibody present in the sample and at least one peptide present in the immunoassay composition.
2. The method of claim 1 , wherein the polynucleotide fragment includes the alanine codon at positions 1117-1119 of SEQ ID NO: 1.
3. The method of claim 1 , wherein the polynucleotide fragment includes the asparagine codon at positions 2518-2520 of SEQ ID NO: 1.
4. The method of claim 1 , wherein the polynucleotide fragment includes the arginine codon at positions 4018-4020 of SEQ ID NO: 1.
5. The method of claim 1 , wherein the polynucleotide fragment includes the glycine codon at positions 4462-4464 and/or and the glutamic acid codon at positions 4465-4467 of SEQ ID NO: 1.
6. The method of claim 1 , wherein the polynucleotide fragment includes the proline codon at positions 6097-6099 and/or the glutamic acid codon at positions 6172-6174 of SEQ ID NO: 1.
7. The method of claim 1 , wherein the polynucleotide fragment includes the serine codon at positions 7840-7842 of SEQ ID NO: 1.
8. The method of claim 1 , wherein the polynucleotide fragment includes the asparagine codon at positions 8518-8520 of SEQ ID NO: 1.
9. The method of claim 1 , wherein the polynucleotide fragment includes the alanine codon at positions 8794-8796 of SEQ ID NO: 1.
10. The method of claim 1 , wherein the immunological reaction between an antibody present in the sample and at least one peptide present in the immunoassay composition is detected by a method comprising EIA.
11. The method of claim 1 , wherein the immunological reaction between an antibody present in the sample and at least one peptide present in the immunoassay composition is detected by a method comprising ELISA.
12. The method of claim 1 , wherein the immunological reaction between an antibody present in the sample and at least one peptide present in the immunoassay composition is detected by a method comprising RIA.
13. The method of claim 1 , wherein the immunological reaction between an antibody present in the sample and at least one peptide present in the immunoassay composition is detected by a method comprising immunofluorescence.