IP Library Granted Patent US 9,670,248
Granted Patent B2
US 9,670,248 · App. 14/170,164 · Granted Jun 6, 2017

Method and system for processing of aquatic species

Inventors: Laurent Olivier (Vero Beach, FL); Greg Havemann (Melbourne, FL); Paul Antalik (Palm Bay, FL); Brandi Alderson (Melbourne, FL)
Assignee: PARABEL LTD.
C07K1/36A23J1/006A23K1/103A23K1/1631A23K1/1643A23L1/3255A23N17/002C08B37/0003Y02E50/10Y02E50/30
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 9,670,248
App. No.
14/170,164
Granted
Jun 6, 2017
Kind
B2
Abstract

The present disclosure relates, according to some embodiments, to methods for recovering multiple products from industrial-scale production of a biomass of an aquatic species. Specifically, the present disclosure relates, in some embodiments, to methods for cultivating Lemna for extracting a protein concentrate, a biocrude, and/or a carbohydrate-rich meal. In some embodiments, a process of recovering at least one product from biomass of an aquatic species may comprise: lysing a biomass to generate a lysed biomass; separating the lysed biomass to generate a juice and a first solid phase; filtering the juice to generate a filtered juice and a second solid phase; clarifying at least one juice to generate a clarified juice and a third solid phase, wherein the clarified juice comprises a soluble protein; coagulating the soluble protein from the clarified juice to generate a liquor comprising a wet protein concentrate; and separating the wet protein concentrate from the liquor.

Claims (41)

1. A process of recovering at least one product from biomass of an aquatic species comprising:

lysing a biomass to generate a lysed biomass;

separating the lysed biomass to generate a juice and a first solid phase;

filtering the juice to generate a filtered juice and a second solid phase;

clarifying at least one juice selected from the group consisting of the juice, the filtered juice, or any combination thereof to generate a clarified juice and a third solid phase,

wherein the clarified juice comprises a soluble protein;

coagulating the soluble protein from the clarified juice to generate a liquor comprising a wet protein concentrate; and

separating the wet protein concentrate from the liquor.

2. The process of claim 1 further comprising:

drying the wet protein concentrate to generate a dry protein concentrate.

3. The process of claim 2 , wherein drying the wet protein comprises using a spin flash dryer, a spray dryer, a drum dryer, a flash dryer, a fluid bed dryer, a double drum dryer, a rotary dryer, evaporator, or any combination thereof.

4. The process of claim 2 , wherein the dry protein concentrate comprises at least 50% protein by weight.

5. The process of claim 1 further comprising:

processing at least one solid phase selected from the group consisting of the first solid phase, the second solid phase, the third solid phase, and any combination thereof to generate a wet bio-crude.

6. The process of claim 5 further comprising:

drying the wet bio-crude to generate at least one product selected from a dry bio-crude and a carbohydrate-rich meal.

7. The process of claim 6 , wherein drying the wet bio-crude comprises using a spin flash dryer, a spray dryer, a drum dryer, a flash dryer, a fluid bed dryer, a double drum dryer, a rotary dryer, or any combination thereof.

8. The process of claim 1 , wherein separating the lysed biomass comprises using at least one of a decanter centrifuge, a belt press, a fan press, a rotary press, a screw press, a filter press, and finisher press, or any combination thereof.

9. The process of claim 1 , wherein the aquatic species comprises a species of Lemna.

10. The process of claim 1 , wherein lysing a biomass comprises using at least one of: a ball mill a colloid mill, a knife mill, a hammer mill, a grinding mill, a puree machine, and a filter press, or any combination thereof.

11. The process of claim 1 , wherein filtering the juice comprises a vibratory separator, a vibrating screen filter, a circular vibratory separator, a linear/inclined motion shaker, a decanter centrifuge, a filter press, or any combination thereof.

12. The process of claim 11 , wherein the vibratory separator comprises at least one vibrating screen filter.

13. The process of claim 1 , wherein clarifying at least one juice comprises using a high-speed multi disc stack centrifuge, a micro filtration system, an ultrafiltration system, or any combination thereof.

14. The process of claim 1 further comprising storing the clarified juice in a chilled storage tank.

15. The process of claim 1 , wherein coagulating protein from the clarified juice comprises lowering the pH of the clarified juice.

16. The process of claim 15 , wherein the pH is lowered to below about 6.

17. The process of claim 15 , wherein the pH is lowered to below about 5.

18. The process of claim 15 , wherein the pH is lowered to about 4.5.

19. The process of claim 15 , wherein lowering the pH comprises using at least one acid selected from hydrochloric acid, nitric acid, and sulfuric acid.

20. The process of claim 1 , wherein coagulating protein from the clarified juice comprises using a heat precipitator comprising at least one heat exchanger.

21. The process of claim 1 , wherein coagulating protein from the clarified juice comprises

heating the clarified juice to a first temperature to generate a heated clarified juice; and

cooling the heated clarified juice to a second temperature to form the liquor comprising the wet protein concentrate.

22. The process of claim 21 , wherein the first temperature is from about 40° C. to about 100° C.

23. The process of claim 21 , wherein the second temperature is below about 40° C.

24. The process of claim 21 , wherein the second temperature is below about 30° C.

25. The process of claim 1 , wherein separating the wet protein concentrate from the liquor comprises using a high-speed multi disc stack centrifuge, settling tank, or any combination thereof to form the wet protein concentrate and a supernatant liquor.

26. The process of claim 25 further comprising:

washing the wet protein concentrate with a first wash; and

separating the wet protein concentrate from the first wash.

27. The process of claim 1 , further comprising washing the lysed biomass.

Assignments (7)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 15, 2026
From: LEMNATURE AQUAFARMS CORPORATION
To: LEMNA PRO LTD
Reel/Frame 074866/0871 →
CHANGE OF NAME Recorded Dec 19, 2022
From: PARABEL NUTRITION, INC.
To: LEMNATURE AQUAFARMS CORPORATION
Reel/Frame 062169/0842 →
CHANGE OF NAME Recorded Apr 14, 2020
From: PARABEL LTD.
To: PARABEL NUTRITION, INC.
Reel/Frame 052396/0163 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 20, 2017
From: HAVEMANN, GREG; ANTALIK, PAUL; ALDERSON, BRANDI
To: PA LLC
Reel/Frame 041648/0710 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 20, 2017
From: OLIVIER, LAURENT
To: PA LLC
Reel/Frame 041648/0657 →
ASSET TRANSFER AGREEMENT Recorded Mar 20, 2017
From: PA LLC
To: PARABEL LTD.
Reel/Frame 041653/0744 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 7, 2015
From: HAVEMANN, GREG; ANTALIK, PAUL; ALDERSON, BRANDI
To: PARABEL LTD.
Reel/Frame 037235/0599 →
Continuity (3)
Continuation 13050931 · Mar 17, 2011
Provisional Application 61314736 · Mar 17, 2010
Related Publication 20140221630A1 · Aug 7, 2014