VLPS containing ligands and methods related thereto
This disclosure relates to immunogenic compositions and methods of enhancing an immune response to an antigen. In certain embodiments, the disclosure relates to virus-like carries comprising a TLR5 agonist on the exterior without an antigen.
1. A method of vaccinating a subject comprising administering an antigen wherein the antigen consists of four copies of M2e polypeptide of SEQ ID NO: 1, in combination with a virus-like carrier comprising a flagellin in a membrane-anchored form comprising a polypeptide of SEQ ID NO: 28, wherein the virus-like carrier does not contain the antigen, under conditions such that antibodies to the antigen are produced.
2. The method of claim 1 , wherein the antigen and virus-like carrier are administered intranasally or intramuscularly.
3. An immunogenic composition comprising an antigen wherein the antigen consists of four copies of M2e polypeptide of SEQ ID NO: 1 and a virus like carrier comprising a flagellin in a membrane-anchored form comprising a polypeptide of SEQ ID NO: 28 wherein the virus-like carrier does not contain the antigen.