Methods of preserving and protecting pancreatic beta cells and treating or preventing diabetes by inhibiting NOX-1
Methods to preserve and/or protect beta cell function by contacting a population or preparation of pancreatic cells, beta cells and/or islets with an inhibitor of NADPH oxidase-1 (NOX-1). Methods of treating a subject for diabetes by administering a therapeutically effective amount of a NOX-1 inhibitor to the subject.
1. A method to preserve and/or protect beta cell function comprising contacting a population or preparation of pancreatic cells, beta cells and/or islets with an inhibitor of NADPH oxidase-1 (NOX-1), wherein the NOX-1 inhibitor is a phenothiazine.
2. The method of claim 1 wherein the NOX-1 inhibitor is 2-acetylphenothiazine.
3. The method of claim 1 wherein the pancreatic cells, beta cells and/or islets have been exposed to stressful stimuli.
4. The method of claim 3 wherein the stressful stimuli is selected from the group consisting of inflammation, inflammatory cytokines, high glucose, elevated free fatty acids and combinations thereof.
5. The method of claim 1 wherein the contacting step is in vitro.
6. The method of claim 1 wherein the contacting step is in vivo.
7. The method of claim 1 wherein the contacting step is ex vivo.
8. The method of claim 1 wherein the method further comprises coadministrating with another pharmaceutical agent.
9. The method of claim 1 wherein a preparation of pancreatic cells is contacted.
10. The method of claim 1 wherein a preparation of beta cells is contacted.
11. The method of claim 1 wherein a preparation of islets is contacted.
12. The method of claim 1 wherein the NOX-1 inhibitor is NCTT-1.