Templated molecules and methods for using such molecules
The present invention relates to a method for synthesising templated molecules. In one aspect of the invention, the templated molecules are linked to the template which templated the synthesis thereof. The intion allows the generation of libraries which can be screened for e.g. therapeutic activity.
1. A composition of more than 10 3 different cyclic molecules each comprising a plurality of covalently linked amino acid residues and a polynucleotide identifying the cyclic molecule, each cyclic molecule being covalently linked by means of a covalent linker to said polynucleotide.
2. The composition according to claim 1 , wherein the polynucleotide is a double stranded polynucleotide.
3. The composition according to claim 1 , wherein the cyclic molecule is selected from the group consisting of α-peptides, β-peptides, γ-peptides and 107 -peptides.
4. The composition according to claim 1 , wherein the covalently linked, amino acid residues are selected from natural amino acids and non-natural amino acids, including a combination of both.
5. The composition according to claim 1 , wherein the cyclic molecule comprises amino acids selected from the group consisting of α-amino acids, β-amino acids, γ-amino acids and ω-amino acids.
6. The composition according to claim 1 , wherein the covalently linked, amino acid residues form a peptide having more than one portion, wherein at least one portion of the peptide is selected from the group consisting of an α-peptide portion, a β-peptide portion, a γ-peptide portion, and a 107 -peptide portion.
7. The composition according to claim 1 , wherein the cyclic molecule comprises L-form amino acids and D-form amino acids.
8. The composition according to claim 1 , wherein the cyclic molecule and the polynucleotide identifying said cyclic molecule are linked by a single linker.
9. The composition according to claim 1 , wherein the cyclic molecule contains from 2 to 10 amino acid residues.
10. The composition according to claim 1 , wherein the cyclic molecule contains from 3 to 8 amino acid residues.
11. The composition according to claim 1 , wherein the cyclic molecule contains from 4 to 6 amino acid residues.
12. The composition according to claim 1 , wherein the cyclic molecule is a monofunctional, difunctional, trifunctional or polyfunctional heterocycle.
13. The composition according to claim 1 , wherein the cyclic molecule is a monocyclic, bicyclic, tricyclic or polycyclic heterocycle.
14. The composition according to claim 1 , wherein the cyclic molecule is a bridged, polycyclic heterocycle.