Molecularly imprinted polymers having affinity for natriuretic peptides
The present invention concerns molecularly imprinted polymers (MIPs) having an affinity for natriuretic peptides, such as atrial natriuretic peptide (ANP). In some embodiments, the MIP is a nanoparticle (a molecularly imprinted polymeric nanoparticle (MIPNP)). Other aspects of the invention include methods of preparing an MIP having affinity for a natriuretic peptide, methods for binding a natriuretic peptide in vitro or in vivo using an MIP of the invention, methods for interfering with the binding of a natriuretic peptide with its receptor in vivo, methods for reducing inflammation, cell growth, cell differentiation, or a cell proliferation disorder, methods for detecting natriuretic peptides, and devices and kits for sequestering and/or detecting natriuretic peptides.
1. A composition of matter, comprising a molecularly imprinted polymer (MIP) nanoparticle having binding affinity for an atrial natriuretic peptide comprising amino acid sequence SLRRSSCFGGRMDRIGAQSGLGCNSFRY (SEQ ID NO: 3), wherein the MIP nanoparticle comprises; (i) methacrylic acid (MAA), N-isopropylacrylamide (NIPAm), and N, N′-methylenebisacrylamide (BIS) in a molar ratio of MMA:NIPAm:BIS of 1:3:10, and (ii) an imprint that possesses steric and chemical memory for amino acid sequence SLRRSS (SEQ ID NO: 2) of the atrial natriuretic peptide, and wherein the MIP nanoparticle selectively binds to SEQ ID NO: 2 of the atrial natriuretic peptide comprising amino acid SEQ ID NO: 3.
2. The composition of matter of claim 1 , wherein the composition of matter is a pharmaceutical composition comprising one or more of the MIP nanoparticles and a pharmaceutically acceptable carrier.
3. The composition of matter of claim 1 , wherein the MIP nanoparticle further comprises a detectable moiety associated with the MIP nanoparticle.