Polysaccharide based hydrogels
Polysaccharide based hydrogel compositions and methods of making and using the same are provided. The subject polysaccharide based hydrogel compositions are prepared by combining a polysaccharide component with a hydrophilic polymer and a cross-linking agent. Also provided are kits and systems for use in preparing the subject compositions.
1. A hydrogel composition, comprising
carboxymethylchitosan having a molecular weight of less than or equal to 5,000 Dalton, a degree of deacetylation of 70% to 99%, an electrophilic group, and at least two nucleophilic groups, wherein carboxymethylchitosan is soluble in an aqueous solution;
a multifunctional, multi-armed polyethylene glycol polymer of a molecular weight of 5,000 Dalton to 30,000 Dalton, and comprising at least two nucleophilic groups; and
a multifunctional, multi-armed crosslinker of polyethylene glycol having a molecular weight of 5,000 Dalton to 30,000 Dalton, and comprising at least two electrophilic groups;
wherein the multifunctional, multi-armed polyethylene glycol polymer comprises ester groups to provide for degradation via hydrolysis and
wherein the hydrogel composition comprises:
a covalent bond between carboxymethylchitosan and the multifunctional multi-armed polyethylene glycol polymer;
a covalent bond between carboxymethylchitosan and the multifunctional multi-armed crosslinker and
a covalent bond between the multifunctional multi-armed polyethylene glycol polymer and the multifunctional multi-armed crosslinker.
2. The composition of claim 1 , wherein carboxymethylchitosan is present in the composition from 0.5% to 10% by mass.
3. The composition of claim 1 , further comprising one or more of a thickening agent, filler, a foaming agent, a biologically active agent, a pharmaceutically active agent, a visualization agent, or a radiopaque agent.
4. The composition of claim 3 , wherein the composition comprises a biologically active agent.
5. The composition of claim 4 , wherein the biologically active agent is a plasma protein, hormone, enzyme, antiseptic agent, antibiotic, antineoplastic agent, antifungal agent, antiviral agent, anti-inflammatory agent, human or non-human growth factor, anesthetic, cell suspension, cytotoxins, cell proliferation inhibitors, fibrin, fibrinogen, collagen or biomimetic.
6. The composition of claim 3 , wherein the composition comprises a filler selected from the group consisting of hydroxyapatite, fibrous silk, carbon fiber, bone chips and a mesh of polyglycolic acid.
7. The composition of claim 3 , wherein the composition comprises a visualization agent.
8. The composition of claim 3 , wherein the composition comprises a radiopaque agent.
9. A hydrogel composition, comprising:
dicarboxyl chitosan having a molecular weight of less than or equal to 5,000 Dalton, a degree of deacetylation of 70% to 99%, an electrophilic group, and at least two nucleophilic groups, wherein the dicarboxyl chitosan is soluble in an aqueous solution;
a multifunctional, multi-armed polyethylene glycol polymer of a molecular weight of 5,000 Dalton to 30 , 000 Dalton, and comprising at least two nucleophilic groups; and
a multifunctional, multi-armed crosslinker of polyethylene glycol having a molecular weight of 5,000 Dalton to 30,000 Dalton, and comprising at least two electrophilic groups;
wherein the multifunctional, multi-armed polyethylene glycol polymer comprises ester groups to provide for degradation via hydrolysis and
wherein the hydrogel composition comprises:
a covalent bond between dicarboxyl chitosan and the multifunctional multi-armed polyethylene glycol polymer;
a covalent bond between dicarboxyl chitosan and the multifunctional multi-armed crosslinker and
a covalent bond between the multifunctional multi-armed polyethylene glycol polymer and the multifunctional multi-armed crosslinker.
10. The composition of claim 9 , wherein dicarboxyl chitosan is present in the composition from 0.5% to 10% by mass.
11. The composition of claim 9 , further comprising one or more of a thickening agent, filler, a foaming agent, a biologically active agent, a pharmaceutically active agent, a visualization agent, or a radiopaque agent.
12. The composition of claim 11 , wherein the composition comprises a biologically active agent.
13. The composition of claim 12 , wherein the biologically active agent is a plasma protein, hormone, enzyme, antiseptic agent, antibiotic, antineoplastic agent, antifungal agent, antiviral agent, anti-inflammatory agent, human or non-human growth factor, anesthetic, cell suspension, cytotoxins, cell proliferation inhibitors, fibrin, fibrinogen, collagen or biomimetic.
14. The composition of claim 11 , wherein the composition comprises a filler selected from the group consisting of hydroxyapatite, fibrous silk, carbon fiber, bone chips and a mesh of polyglycolic acid.
15. The composition of claim 11 , wherein the composition comprises a visualization agent.
16. The composition of claim 11 , wherein the composition comprises a radiopaque agent.