Compositions and methods for treating and preventing tissue injury and disease
The present invention provides novel compositions comprising multipotent cells or microvascular tissue, wherein the cells or tissue has been sterilized and/or treated to inactivated viruses, and related methods of using these compositions to treat or prevent tissue injury or disease in an allogeneic subject.
1. A method of preparing a composition comprising processed microvascular tissue, wherein the composition has angiogenic or anti-inflammatory activity, the method comprising:
(a) dissociating a microvascular tissue sample obtained from a donor mammal to produce a composition comprising dissociated microvascular tissue;
(b) removing one or more tissue components from the composition produced according to (a);
(c) optionally drying, lyophilizing, or cryopreserving the composition following (b) or (d); and
(d) sterilizing the composition following (b) or (c) and/or inactivating virus present in the composition following (b) or (c);
wherein the composition resulting from (c) or (d) retains measurable angiogenic or anti-inflammatory activity;
wherein:
(i) the cells or tissue are not cultured;
(ii) the method further comprises filtering the composition;
(iii) the dissociation of (a) comprises contacting the tissue sample with one or more proteases; and
(iv) the dissociation of (a) or the removing of (b) comprises ultrasonic agitation, centrifugation, filtration, or use of a density gradient; and further wherein
(v) the one or more proteases does not comprise collagenase;
(vi) the one or more proteases comprises or consists of: collagenase type 1 and either dispase or thermolysin; or MMP 2, MMP 14 and either dispase or thermolysin; or
(vii) the microvascular tissue is adipose-derived tissue, and the ultrasonic agitation, centrifugation, filtration or use of a density gradient removes adipocytes from the composition produced according to (a).