Dose selection of adjuvanted synthetic nanocarriers
Disclosed are synthetic nanocarrier compositions with coupled adjuvant compositions as well as related methods.
1. A method comprising:
providing a dose of adjuvant and a dose of antigen, wherein at least a portion of the dose of adjuvant is coupled to synthetic nanocarriers,
generating an antibody titer against the antigen through administration of the dose of adjuvant and the dose of antigen to a subject, and
choosing the dose of adjuvant to be less than a separate dose of adjuvant that results in an antibody titer similar to that generated through administration of the dose of adjuvant and the dose of antigen to the subject.
2. The method of claim 1 , wherein the adjuvant comprises an agonist for Toll-Like Receptors 3, 4, 5, 7, 8, or 9 or a combination thereof.
3. The method of claim 2 , wherein the adjuvant comprises an agonist for Toll-Like Receptors 3, an agonist for Toll-Like Receptors 7 and 8, or an agonist for Toll-Like Receptor 9.
4. The method of claim 3 , wherein the adjuvant comprises R848, immunostimulatory DNA, or immunostimulatory RNA.
5. The method of claim 1 , wherein the dose of adjuvant comprises two or more types of adjuvants.
6. The method of claim 1 , wherein a portion of the dose of adjuvant is not coupled to the synthetic nanocarriers.
7. The method of claim 1 , wherein more than one type of antigen are administered to the subject.
8. The method of claim 1 , wherein at least a portion of the dose of antigen(s) is coupled to the synthetic nanocarriers.
9. The method of claim 1 , wherein at least a portion of the dose of antigen(s) is not coupled to the synthetic nanocarriers.
10. The method of claim 1 , wherein at least a portion of the dose of antigen(s) is coadministered with the synthetic nanocarriers.
11. The method of claim 1 , wherein at least a portion of the dose of antigen(s) is not coadministered with the synthetic nanocarriers.
12. The method of claim 1 , wherein the antigen(s) comprise a B cell antigen and/or a T cell antigen.
13. The method of claim 12 , wherein the T cell antigen comprises a T-helper cell antigen.
14. The method of claim 1 , wherein the antigen(s) comprise a B cell antigen or a T cell antigen and a T-helper cell antigen.
15. The method of claim 1 , wherein the administration is by a route that comprises subcutaneous, intramuscular, intradermal, oral, intranasal, transmucosal, rectal;
ophthalmic, transdermal or transcutaneous administration, or a combination thereof.
16. The method of claim 1 , wherein the synthetic nanocarriers comprise lipid nanoparticles, polymeric nanoparticles, metallic nanoparticles, surfactant-based emulsions, dendrimers, buckyballs, nanowires, virus-like particles, peptide or protein particles, nanoparticles that comprise a combination of nanomaterials, spheroidal nanoparticles, cuboidal nanoparticles, pyramidal nanoparticles, oblong nanoparticles, cylindrical nanoparticles, or toroidal nanoparticles.
17. The method of claim 1 , wherein the synthetic nanocarriers comprise one or more polymers.
18. The method of claim 17 , wherein the one or more polymers comprise a polyester.