IP Library Granted Patent US 9,765,293
Granted Patent B2
US 9,765,293 · App. 14/415,744 · Granted Sep 19, 2017

Agglomerated microbiological media

Inventors: Jie J. Liu (Woodbury, MN); Wensheng Xia (Woodbury, MN)
Assignee: 3M INNOVATIVE PROPERTIES COMPANY
C12N1/20C12N1/14C12N1/16C12N2500/46C12N2500/60
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Quick Facts
Patent No.
US 9,765,293
App. No.
14/415,744
Granted
Sep 19, 2017
Kind
B2
Abstract

A method of making a flowable, dried agglomerated nutrient medium is provided. The method comprises introducing a nutrient component comprising a powdered nutrient, and an agglomeration liquid, into an agglomerator comprising a flow-through-type agglomeration chamber, wet-massing the nutrient component with the agglomeration liquid in the agglomeration chamber for a predetermined period of time to form agglomerated nutrient medium particles, and exposing the agglomerated nutrient medium particles to drying conditions for a period of time to form the dried, agglomerated nutrient medium. The nutrient component facilitates the growth of a microorganism. Compositions, articles, and kits comprising the flowable, dried agglomerated nutrient medium are also provided.

Claims (30)

1. A method of making a flowable, dried agglomerated nutrient medium, the method comprising:

introducing a nutrient component comprising a powdered nutrient, and an agglomeration liquid, into an agglomerator comprising a flow-through-type agglomeration chamber and a mixing blade;

wherein the nutrient component facilitates the growth of a microorganism;

wherein the agglomeration liquid is introduced as an atomized spray;

wherein introducing the nutrient component into the agglomeration chamber comprises introducing the nutrient component at a feed rate of about 50 kg/hour to about 1000 kg/hour;

wherein introducing the agglomeration liquid into the agglomeration chamber comprises introducing the agglomeration liquid at a spray rate about 15 kg/hour to about 300 kg/hour;

wet-massing the nutrient component with the agglomeration liquid in the agglomeration chamber for a predetermined period of time to form agglomerated nutrient medium particles;

exposing the agglomerated nutrient medium particles to drying conditions for a period of time to form the dried, agglomerated nutrient medium;

wherein the mixing blade is rotated at a blade rotation rate of about 500 rpm to about 4000 rpm;

wherein exposing the agglomerated nutrient medium to drying conditions comprises exposing the agglomerated nutrient medium to a predetermined temperature about 60° C. to about 85° C.;

wherein the feed rate, the spray rate, the blade rotation rate, and the predetermined temperature are selected to produce a dried agglomerated nutrient medium having particles of a first particle size distribution range of about 105 microns to about 2000 microns; and

isolating a subpopulation of the particles, the subpopulation having a second particle size distribution range that is narrower than the first particle size distribution range, the second particle size distribution range being about 149 microns to 1000 microns.

2. The method of claim 1 , wherein the agglomeration liquid consists of water.

3. The method of claim 1 , wherein the agglomeration liquid comprises a solvent having a dissolved nutrient that facilitates the growth of a microorganism.

4. The method of claim 1 , wherein introducing a nutrient component comprises introducing a substantially uniform mixture of two or more powdered nutrients.

5. The method of claim 1 :

wherein introducing a nutrient component into the agglomeration chamber comprises introducing the nutrient component at a feed rate of about 50 kg/hour to about 1000 kg/hour;

wherein introducing an agglomeration liquid into the agglomeration chamber comprises introducing the agglomeration liquid at a spray rate of about 15 kg/hour to about 300 kg/hour;

wherein the agglomerator further comprises a mixing blade, wherein the mixing blade is rotated at a blade rotation rate of about 500 rpm to about 4000 rpm;

wherein exposing the agglomerated nutrient medium to drying conditions comprises exposing the agglomerated nutrient medium to a predetermined temperature of about 60° C. to about 85° C.;

wherein the feed rate, the spray rate, the blade rotation rate, and the predetermined temperature are selected to produce a dried agglomerated nutrient medium having a preselected mean water content of less than 5 weight percent.

6. The method of claim 1 :

wherein introducing a nutrient component into the agglomeration chamber comprises introducing the nutrient component at a feed rate of about 50 kg/hour to about 1000 kg/hour;

wherein introducing an agglomeration liquid into the agglomeration chamber comprises introducing the agglomeration liquid at a spray rate about 15 kg/hour to about 300 kg/hour;

wherein the agglomerator further comprises a mixing blade, wherein the mixing blade is rotated at a blade rotation rate of about 500 rpm to about 4000 rpm;

wherein exposing the agglomerated nutrient medium to drying conditions comprises exposing the agglomerated nutrient medium to a predetermined temperature about 60° C. to about 85° C.;

wherein the feed rate, the spray rate, the blade rotation rate, and the predetermined temperature are selected to produce a dried agglomerated nutrient medium having a gravity-packed density of 0.2 to 0.5 grams per cubic centimeter.

7. The method of claim 1 , wherein one or more powdered nutrient is selected from the group consisting of a protein, a carbohydrate, a salt and a mixture of any two or more of the foregoing powdered nutrients.

8. The method of claim 1 , further comprising the step of subjecting the dried agglomerated nutrient medium to a process that reduces the number of viable microorganisms in the dried agglomerated nutrient medium.

9. The method of claim 8 , wherein subjecting the dried agglomerated nutrient medium to a process that reduces the number of viable microorganisms comprises exposing the dried agglomerated nutrient medium to ionizing radiation or to ethylene oxide vapor.

Assignments (4)
SECURITY INTEREST Recorded Sep 2, 2022
From: NEOGEN CORPORATION; NEOGEN FOOD SAFETY US HOLDCO CORPORATION
To: JPMORGAN CHASE BANK, N.A., AS COLLATERAL AGENT
Reel/Frame 061372/0264 →
PATENT ASSIGNMENT AGREEMENT Recorded Sep 1, 2022
From: 3M INNOVATIVE PROPERTIES COMPANY
To: GARDEN US HOLDCO CORPORATION
Reel/Frame 061365/0688 →
CHANGE OF NAME Recorded Sep 1, 2022
From: GARDEN US HOLDCO CORPORATION
To: NEOGEN FOOD SAFETY US HOLDCO CORPORATION
Reel/Frame 061370/0437 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 28, 2015
From: LIU, JIE J.; XIA, WENSHENG
To: 3M INNOVATIVE PROPERTIES COMPANY
Reel/Frame 034827/0511 →
Continuity (2)
Provisional Application 61675624 · Jul 25, 2012
Related Publication 20150191692A1 · Jul 9, 2015