Repebody against immunoglobulin G and uses thereof
The present invention relates to a polypeptide (repebody) selectively bound to an immunoglobulin G, a polynucleotide which encodes the repebody, a vector containing the polynucleotide, a recombinant microorganism in which the polynucleotide is introduced, a method for producing the repebody using the recombinant microorganism, and a method for immobilizing or purifying an immunoglobulin G using the repebody. The repebody according to the present invention is useful as utilized for immobilization of an immunoglobulin G, purification of an immunoglobulin G, and production of an immunosensor, since the repebody selectively bound to an immunoglobulin G.
1. A repebody consisting of the amino acid sequence of SEQ ID NO: 3, wherein the repebody is capable of selectively binding to immunoglobulin G.
2. A repebody consisting of the amino acid sequence of SEQ ID NOs: 4 or 5, wherein the repebody is capable of selectively binding to immunoglobulin G.
3. A polynucleotide which encodes the repebody of claim 1 .
4. A vector which contains the polynucleotide of claim 3 .
5. A recombinant microorganism, in which the polynucleotide of claim 3 , or a vector containing said polynucleotide, is introduced.
6. A method for producing the repebody of claim 1 , wherein the method comprises:
(i) expressing the repebody by culturing the recombinant microorganism of claim 5 ; and
(ii) recovering the expressed repebody.