Composition of matter comprising library of first nucleic acid analyte copies
This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
1. A composition of matter comprising:
a library of nucleic acid copies of different nucleic acid analytes,
said copies having been copied from different nucleic acid analytes comprising genomic DNA, episomal DNA, mRNA or a combination of any of the forgoing isolated or derived from a eukaryotic cell, and
said copies comprising an inherent universal detection target (UDT) derived from the different nucleic acid analytes that have been copied;
an array comprising different nucleic acids located at different locations of the array,
said nucleic acids being fixed or immobilized to a solid support, and
nucleic acid copies of the library which have complementarity to a nucleic acid of a location of the array being hybridized to the nucleic acid of the array at said location; and
a universal detection element (UDE) bound to said UDT of nucleic acid copies hybridized to nucleic acids of said array,
wherein said UDT comprises a secondary structure,
wherein the binding of the UDE to the UDT is mediated by said secondary structure of the UDT, and
wherein said UDE generates a signal directly or indirectly to detect the presence or quantity of said analyte copies.
2. The composition of claim 1 , wherein said nucleic acid array comprises DNA, DNA analogs, RNA, RNA analogs, or a combination thereof.
3. The composition of claim 2 , wherein said nucleic acid array comprises analogs comprising PNA.
4. The composition of claim 2 , wherein said DNA, RNA or analogs are modified on the sugar moiety.
5. The composition of claim 1 , wherein said solid support is porous.
6. The composition of claim 5 , wherein said porous solid support comprises polyacrylamide or agarose.
7. The composition of claim 1 , wherein said solid support is nonporous.
8. The composition of claim 7 , wherein said non-porous solid support comprises glass or plastic.
9. The composition of claim 1 , wherein said solid support is transparent, translucent, opaque or reflective.
10. The composition of claim 1 , wherein said nucleic acids are directly fixed or immobilized to said solid support.
11. The composition of claim 1 , wherein said nucleic acids are indirectly-fixed or immobilized to said solid support.
12. The composition of claim 11 , wherein said nucleic acids are indirectly fixed or immobilized to said solid support by means of a chemical linker or linkage arm.
13. The composition of claim 1 , wherein said UDE comprises nucleic acids, nucleic acid analogs, polypeptides, polysaccharides, synthetic polymers, or a combination of any of the foregoing.
14. The composition of claim 13 , wherein said UDE comprises nucleic acid analogs, wherein the nucleic acid analogs comprise PNA.
15. The composition of claim 1 , wherein said UDE comprises direct signal generating means and generates a signal directly.
16. The composition of claim 15 , wherein said direct signal generating means comprises a compound selected from the group consisting of fluorescent compound, a phosphorescent compound, a chemiluminescent compound, a chelating compound, an electron dense compound, a magnetic compound, an intercalating compound, an energy transfer compound, or a combination of any of the foregoing.
17. The composition of claim 1 , wherein said UDE generates a signal indirectly by indirect signal generating means.
18. The composition of claim 17 , wherein said indirect signal generating means comprises an antibody, an antigen, a hapten, a receptor, a hormone, a ligand, an enzyme, or a combination of any of the foregoing.
19. The composition of claim 18 , wherein said indirect signal generation comprises an enzyme, wherein the enzyme catalyzes a reaction comprising a fluorogenic reaction, a chromogenic reaction or a chemiluminescent reaction.
20. The composition of claim 2 , wherein said DNA, RNA or analogs are modified on the phosphate moiety.
21. The composition of claim 2 , wherein said DNA, RNA or analogs are modified on the base moiety.
22. The composition of claim 1 , wherein the nucleic acid copies are unlabeled.