CA6 antigen-specific cytotoxic conjugate and methods of using the same
Cytotoxic conjugates comprising a cell binding agent and a cytotoxic agent, therapeutic compositions comprising the conjugate, methods for using the conjugates in the inhibition of cell growth and the treatment of disease, and a kit comprising the cytotoxic conjugate are disclosed are all embodiments of the invention. In particular, the cell binding agent is a monoclonal antibody, and epitope-binding fragments thereof, that recognizes and binds the CA6 glycotope. The present invention is also directed to humanized or resurfaced versions of DS6, an anti-CA6 murine monoclonal antibody, and epitope-binding fragments thereof.
1. A polynucleotide comprising a nucleotide sequence encoding the variable heavy chain of SEQ ID NO:11.
2. A polynucleotide comprising a nucleotide sequence encoding the variable heavy chain of SEQ ID NO:10.
3. A polynucleotide comprising a nucleotide sequence encoding the variable light chain of SEQ ID NO:8.
4. A vector comprising the polynucleotide of claim 1 .
5. A vector comprising the polynucleotide of claim 2 .
6. A vector comprising the polynucleotide of claim 3 .
7. A vector comprising the polynucleotide of claim 1 and a polynucleotide comprising a nucleotide sequence encoding the variable light chain of SEQ ID NO:8.
8. A host cell comprising the polynucleotide of claim 1 .
9. A host cell comprising the polynucleotide of claim 2 .
10. A host cell comprising the polynucleotide of claim 3 .
11. A host cell comprising the polynucleotide of claim 1 and a polynucleotide comprising a nucleotide sequence encoding the variable light chain of SEQ ID NO:8.
12. A host cell comprising the vector of claim 4 .
13. A host cell comprising the vector of claim 5 .
14. A host cell comprising the vector of claim 6 .
15. A host cell comprising the vector of claim 7 .
16. A host cell comprising the vector of claim 5 and a vector comprising a polynucleotide comprising a nucleotide sequence encoding the variable light chain of SEQ ID NO:8.
17. The host cell of claim 11 , wherein the host cell is a CHO cell.
18. The host cell of claim 15 , wherein the host cell is a CHO cell.
19. The host cell of claim 16 , wherein the host cell is a CHO cell.
20. A vector comprising the polynucleotide of claim 2 and a polynucleotide comprising a nucleotide sequence encoding the variable light chain of SEQ ID NO:8.