Inactivated chikungunya viruses (CHIKV) comprising an E1-K211E mutation
A vaccine composition for prophylaxis and treatment of Chikungunya virus infections is disclosed which is capable of conferring immunity against any genotypic variants of the Chikungunya virus. More particularly the invention discloses particular nucleotide sequences and their translated proteins thereof, which may be expressed as Virus Like Particles which for use as a vaccine antigens against Chikungunya virus infections. The compositions disclosed in this invention are also protective against any genotypic variants of the Chikungunya virus which may be propagated by any suitable vector of the disease including Aedis albopictus and Aedis aegypti.
1. A composition comprising inactivated Chikungunya virus strain wherein the Chikungunya virus strain has a non-synonymous mutation K1020E in the structural polyprotein corresponding to E1-K211E in the E1 structural glycoprotein either singly or in combination with other mutations selected from the group consisting of (i) K1020E of E1-211 protein in combination with, G380R of E2-55 protein, V589A of E2-264 protein in chikungunya virus strain TN01610; (ii) K1020E of E1-211 protein in combination with, D301N of E3-40 protein, V589A of E2-264 protein, G1004R of E1-195 protein in chikungunya virus strain TN151100; (iii) K1020E of E1-211 protein in combination with, K372N of E2-47 protein, V589A of E2-264 protein in chikungunya virus strain TN06210; (iv) K1020E of E1-211 protein in combination with, A232V of C-232 protein, V589A of E2-264 protein, G1004R of E1-195 protein in chikungunya virus strain TN06310; (v) K1020E of E1-211 protein in combination with, K391E of E2-66 protein, V589A of E2-264 protein in chikungunya virus strain TN06410; and (vi) K1020E of E1-211 protein in combination with, G380R of E2-55 protein, P867L of E1-58 protein, in chikungunya virus strain AP0109.
2. The composition of claim 1 , wherein the inactivated Chikungunya virus strains contain nucleotide sequences disclosed in SEQ. ID. NO:1 to SEQ ID NO:7 and SEQ ID NO:15 to SEQ ID NO:20.
3. The composition of claim 1 , wherein the composition further comprises an adjuvant selected from the group consisting of aluminum; hydroxide; aluminum phosphate;
gamma inulin; algammulin; cholecalciferol in oil; an oil in water emulsion containing squalene, tween-80, andSpan-85 in 10 mM phosphate-citrate buffer; oil in water emulsion containing squalene, tween-80, Span-85, alpha tocopherol in phosphate-citrate buffer; an oil in water emulsion containing squalene, tween-80, Span-85 and cholecalciferol.
4. The composition of claim 1 , wherein the inactivated Chikungunya virus strain has a non-synonymous mutation K1020E in the structural polyprotein corresponding to E1-K211E in the E1 structural glycoprotein in combination with other mutations selected from the group consisting of SEQ ID NO:1 to SEQ ID NO:7 and SEQ ID No:15 to SEQ ID No:20.
5. The composition of claim 1 further comprising an inactivated Japanese encephalitis antigen.