Optical method and device for identifying and quantifying analytes
A method for analyzing a metalloprotein and/or the interaction with its environment comprising the following steps: (a) Providing a medium that enhances the detection of the electromagnetic cross-section signal of metalloproteins, (b) Incorporating a metalloprotein to analyse into said medium, (c) Contacting said medium with electromagnetic radiation, (d) Obtaining the electromagnetic cross-section spectrum of said metalloprotein, (e) Determining from said electromagnetic cross-section spectrum at least one parameter related to one or several analytes of interest.
1. A method for analyzing an analyte comprising the steps of:
(a) providing a multiscattering medium that enhances a detection of an electromagnetic cross-section signal of a metalloprotein,
(b) incorporating the metalloprotein to be analyzed into the multiscattering medium and the analyte is arranged to establish a redox reaction with the metalloprotein,
(c) contacting the multiscattering medium with electromagnetic radiation,
(d) obtaining the electromagnetic cross-section spectrum of the metalloprotein, and
(e) determining from the electromagnetic cross-section spectrum a concentration of the analyte.
2. The method according to claim 1 wherein the electromagnetic cross-section is an absorption cross-section of the metalloprotein.
3. The method according to claim 1 wherein the multiscattering medium is at least one of an aggregate; a suspension; a gel; a porous membrane; a microstructured substrate; and a nanostructured substrate.
4. The method according to claim 1 wherein the analyte is a reaction product of enzymatic or chemical reactions.
5. The method according to claim 1 wherein the step of (c) contacting uses a dark-field electromagnetic radiation illumination.
6. The method according to claim 1 wherein the step of (e) determining further includes:
determining the concentration of the analyte based on a measurement of a molecular state of the metalloprotein.
7. The method according to claim 6 wherein the molecular state is an oxidation state.
8. The method according to claim 1 wherein the analyte is hydrogen peroxide.
9. The method according to claim 1 wherein the metalloprotein is a cytochrome c.
10. The method according to claim 1 , wherein the multiscattering medium enhances an optical cross-section of the metalloprotein.
11. The method according to claim 1 , wherein the multiscattering medium provides for an amplification of the electromagnetic cross-section spectrum to suppress a background signal.