Plurality of reaction chambers in a test cartridge
A fluidic testing system is presented that includes a plurality of test chambers, a plurality of inlet channels, and a fluidic network that connects the inlet channels to one or more other chambers. The plurality of test chambers are each characterized by a length and a hydraulic diameter. The length of each test chamber is aligned substantially parallel to a gravity vector. Each of the test chambers has only one opening disposed along the length of the corresponding test chamber. Additionally, each of the test chambers is coupled via its respective opening to only one of the plurality of inlet channels.
1. A method comprising:
flowing a first liquid through a first inlet channel coupled to a first opening disposed along a length of a test chamber at a first height, wherein the length of the test chamber is configured to be aligned substantially parallel to a gravity vector;
filling the test chamber with the first liquid up to a first threshold amount;
drawing the first liquid away from the test chamber through the first inlet channel, wherein the drawing leaves behind a first predetermined amount of the first liquid within the test chamber;
flowing a second liquid through a second inlet channel coupled to a second opening disposed along the length of the test chamber at a second height, wherein the second height is greater than the first height;
filling the test chamber with the second liquid up to a second threshold amount;
drawing the second liquid away from the test chamber through the second inlet channel, wherein the drawing leaves behind a second predetermined amount of the second liquid within the test chamber.
2. The method of claim 1 , wherein leaving behind a first predetermined amount of the first liquid, comprises leaving behind an amount of the first liquid that corresponds to the first height of the first opening.
3. The method of claim 2 , wherein leaving behind a second predetermined amount of the second liquid comprises leaving behind an amount of the second liquid that corresponds to a difference between the second height of the second opening and the first height of the first opening.
4. The method of claim 1 , wherein the test chamber contains one or more reagents, and the method further comprises re-suspending the one or more reagents in the test chamber in the predetermined amount of the first and second liquid left behind within the test chamber.
5. The method of claim 1 , wherein the test chamber contains one or more reagents, and the method further comprises re-suspending the one or more reagents in the test chamber in the predetermined amount of only the first liquid left behind within the test chamber.
6. The method of claim 5 , wherein the drawing of either the first or second liquid draws liquid that does not contain any of the one or more reagents.
7. The method of claim 1 , further comprising heating the first liquid and the second liquid within the test chamber.
8. The method of claim 7 , wherein the heating comprises heating with a Peltier device.
9. The method of claim 7 , wherein the heating comprises heating with resistive heating elements.
10. The method of claim 7 , wherein the heating comprises heating with forced air.
11. The method of claim 1 , further comprising detecting one or more optical properties of contents within the test chamber.
12. The method of claim 1 , wherein the flowing of the first and second liquid is performed via a single pump source.
13. The method of claim 1 , further comprising, after the drawing of the first liquid, closing off the first inlet channel from the atmosphere.