NF-κB signaling pathway-manipulated dendritic cells
The invention relates to dendritic cells, the NFκB signaling pathway of which has been manipulated by RNA transfection, to the manufacture thereof and to use thereof.
1. A method for ex vivo manufacture of dendritic cells, the NFκB signaling pathway of which has been manipulated, comprising the RNA-transfection of mature DCs with one or more RNA sequences encoding a mutant signal transducing protein of the NFκB signaling pathway, wherein the mutant signal transducing protein of the NFκB signaling pathway is a constitutively active IKKβ mutant, wherein the amino acid residues Ser177 and Ser181 of the IKKβ wild-type of SEQ ID NO:4 are replaced by Glu, and the destabilizing C-terminal serine residues at positions 670, 672, 675, 679, 682, 689, 692, 695, 697, and 705 of the wild-type of SEQ ID NO:4 are replaced by alanine residues.
2. The method according to claim 1 , wherein the RNA-transfection is carried out by electroporation.
3. The method according to claim 1 , further comprising
(i) loading of the dendritic cells with a target antigen; and/or
(ii) the cryopreservation of matured and transfected dendritic cells.