PD-L1 antibodies and uses thereof
Provided herein are PD-L1 antibodies and methods for using the same for diagnosing a medical condition associated with elevated PD-L1 levels (e.g., cancer) in subjects in need thereof and antigen binding fragments thereof. The PD-L1 antibodies and antigen binding fragments are also useful in evaluating the efficacy of a particular therapeutic regime in a subject diagnosed as having a PD-L1-related medical condition.
1. An isolated antibody comprising a heavy chain (HC) immunoglobulin variable domain sequence and a light chain (LC) immunoglobulin variable domain sequence, wherein the antibody specifically binds to an epitope of human PD-L1comprising the amino acid sequence CGIQDTNSKKQSDTHLEET (SEQ ID NO: 1) in formalin-fixed, paraffin embedded tissue with a half maximal effective concentration (EC 50 ) of at least 1.5×10 −11 M, wherein:
the HC comprises
(a) a HC CDR1 comprising the amino acid sequence NHAIS (SEQ ID NO: 14); and
(b) a HC CDR2 comprising the amino acid sequence TINSDTHTYYATWPKG (SEQ ID NO: 15); and
(c) a HC CDR3 comprising the amino acid sequence RIFSSSNI (SEQ ID NO: 16); and
the LC comprises
(a) a LC CDR1 comprising the amino acid sequence QASQSIYNNNWLS (SEQ ID NO: 17); and
(b) a LC CDR2 comprising the amino acid sequence LASTLAS (SEQ ID NO: 12); and
(c) a LC CDR3 comprising the amino acid sequence IGGESSNNDGIA (SEQ ID NO: 18);
or an antigen binding fragment thereof.
2. The antibody or antigen binding fragment of claim 1 , wherein the HC immunoglobulin variable domain sequence comprises the amino acid sequence of SEQ ID NO: 2.
3. The antibody or antigen binding fragment of claim 1 , wherein the LC immunoglobulin variable domain sequence comprises the amino acid sequence of SEQ ID NO: 3.
4. The antibody or antigen binding fragment of claim 1 , wherein the HC immunoglobulin variable domain sequence comprises the amino acid sequence of SEQ ID NO: 2, and wherein the LC immunoglobulin variable domain sequence comprises the amino acid sequence of SEQ ID NO: 3.
5. The antibody or antigen binding fragment of claim 1 , wherein the antibody is a monoclonal antibody.
6. A composition comprising the antibody or antigen binding fragment of claim 1 bound to a peptide comprising SEQ ID NO: 1.
7. The composition of claim 6 wherein the peptide is a PD-L1 protein.
8. The composition of claim 6 , wherein the peptide is associated with a cell.
9. The composition of claim 6 , wherein the peptide is bound to a solid support.
10. The composition of claim 6 , wherein the peptide is disposed in a solution.
11. The composition of claim 6 , wherein the peptide is associated with a matrix.
12. A kit for detecting PD-L1 comprising an antibody or antigen binding fragment of claim 1 , and instructions for use.