Loci associated with charcoal rot drought complex tolerance in soybean
The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to Charcoal Rot Drought Complex. The methods use molecular genetic markers to identify, select and/or construct tolerant plants or identify and counter-select susceptible plants. Soybean plants that display tolerance or improved tolerance to Charcoal Rot Drought Complex that are generated by the methods of the invention are also a feature of the invention. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
1. A method of breeding a soybean plant tolerant to Charcoal Rot, the method comprising:
a. isolating nucleic acid or tissue containing nucleic acid from a soybean plant;
b. detecting in the nucleic acid the presence of one or more markers associated with Charcoal Rot tolerance,
wherein the one or more markers are located in a chromosomal interval located between 7,975 and 8,015 kbp of chromosome 5, and
wherein said chromosomal interval includes a sequence that hybridizes with the S04257-1 marker at position 7,995,435 of chromosome 5;
c. thereby identifying and/or selecting a Charcoal Rot tolerant soybean plant;
d. crossing the soybean plant identified and/or selected in step c with a second plant not having said one or more markers in said chromosomal interval;
e. collecting seeds from said cross; and
f. growing a progeny soybean plant from said seed which comprises said one or more markers in said chromosomal interval, thereby producing a soybean plant with increased tolerance to Charcoal Rot compared to a soybean plant not comprising said one or more markers in said chromosomal interval.