Patent
The present invention relates to an Agrobacterium having improved gene transfer efficiency. The present invention provides a transformed Agrobacterium which harbors a foreign GABA transaminase gene and exhibits improved gene transfer efficiency, and a method for producing a transformed plant using the same.
1. A transformed Agrobacterium which harbors a foreign γ-aminobutyric acid (GABA) transaminase gene and exhibits improved gene transfer efficiency, wherein the foreign GABA transaminase gene is not present in a T-DNA region.
2. The transformed Agrobacterium according to claim 1 , which further harbors a foreign 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene, wherein the foreign ACC deaminase gene is not present in a T-DNA region.
3. The transformed Agrobacterium according to claim 1 , wherein the transformed Agrobacterium harbors the foreign GABA transaminase gene in a vector.
4. The transformed Agrobacterium according to claim 1 , which further comprises a binary vector comprising a T-DNA region.
5. The transformed Agrobacterium according to claim 1 , wherein the GABA transaminase gene is derived from a bacterium.
6. The transformed Agrobacterium according to claim 2 , wherein the ACC deaminase gene is derived from a bacterium.
7. A method for producing a transformed plant, comprising carrying out gene transfer to a plant using the transformed Agrobacterium according to claim 1 .
8. The method according to claim 7 , wherein the plant is a monocotyledon or a dicotyledon.
9. The method according to claim 8 , wherein the monocotyledon is a plant of the family Poaceae.
10. The method according to claim 8 , wherein the dicotyledon is a plant of the family Solanaceae.
11. The transformed Agrobacterium according to claim 2 , which further comprises a binary vector comprising a T-DNA region.
12. A method for producing a transformed plant, comprising carrying out gene transfer to a plant using the transformed Agrobacterium according to claim 2 .
13. The method according to claim 12 , wherein the plant is a monocotyledon or a dicotyledon.
14. The method according to claim 13 , wherein the monocotyledon is a plant of the family Poaceae.
15. The method according to claim 13 , wherein the dicotyledon is a plant of the family Solanaceae.
16. The transformed Agrobacterium according to claim 2 , wherein the transformed Agrobacterium harbors the GABA transaminase gene and the ACC deaminase gene in a vector.