Method for using hexenol glycosyl transferase
A method for producing a hexenol glycoside using a hexenol glycosyltransferase. A transformant transformed with a gene encoding a hexenol glycosyltransferase and a method for preparing such a transformant.
1. A method for producing a hexenol glycoside, comprising:
contacting an isolated, purified or extracted protein with a UDP-sugar and a hexenol molecule to cause glycosylation of the hexenol molecule and to thereby produce a hexenol glycoside;
wherein the isolated, purified or extracted protein has glycosylation activity on the hexenol molecule and is selected from the group consisting of:
(a) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or 4;
(b) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or 4, except that no more than 1 to 24 amino acids are deleted, substituted, inserted, and/or added; and
(c) a protein comprising an amino acid sequence that has 95% or more sequence identity with the amino acid sequence of SEQ ID NO: 2 or 4.
2. The method according to claim 1 , wherein the UDP-sugar is a UDP-hexose.
3. The method according to claim 2 , wherein the hexose of the UDP-hexose is selected from the group consisting of glucose, mannose and galactose.
4. The method according to claim 1 , further comprising purifying the hexenol glycoside.
5. A method for producing a hexenol glycoside, comprising:
providing a cell comprising a polynucleotide encoding a protein, wherein said cell expresses said protein, and said cell is a microorganism or a transgenic plant cell, and
contacting within or outside of the cell said protein with a UDP-sugar and a hexenol molecule to cause glycosylation of the hexenol molecule and to thereby produce a hexenol glycoside,
wherein said polynucleotide comprises a nucleotide sequence encoding said protein and an expression control region that controls expression of said nucleotide sequence and wherein the nucleotide sequence encoding said protein and/or the expression control region is heterologous to said cell,
wherein said protein has glycosylation activity on the hexenol molecule and is selected from the group consisting of:
(a) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or 4;
(b) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or 4, except that no more than 1 to 24 amino acids are deleted, substituted, inserted, and/or added; and
(c) a protein comprising an amino acid sequence that has 95% or more sequence identity with the amino acid sequence of SEQ ID NO: 2 or 4.
6. The method according to claim 5 , wherein the UDP-sugar is a UDP-hexose.
7. The method according to claim 6 , wherein the hexose of the UDP-hexose is selected from the group consisting of glucose, mannose and galactose.
8. The method according to claim 5 , further comprising purifying the hexenol glycoside.
9. The method according to claim 5 , wherein the transgenic plant cell is from a transformed whole plant, a transformed plant organ, a transformed plant tissue, transformed cultured plant cells, or from a progeny plant of a transgenic whole plant whose genome comprises the polynucleotide encoding the protein having glycosylation activity on the hexenol molecule.