Method of enzyme inactivation
The present invention relates to a method for the inactivation of enzymes with a composition comprising a sulfonated peroxycarboxylic acid according to formula (I) wherein: R 1 is hydrogen, or a substituted or unsubstituted C m alkyl group; R 2 is a substituted or unsubstituted C n alkyl group; X is a hydrogen atom, a cationic group, or an ester forming moiety; Y is a hydrogen atom, a cationic group, or an ester forming moiety; n is 1 to 10; m is 1 to 10; and n+m is less than or equal to 18, wherein the composition is contacted with the enzyme.
1. A method for the inactivation of enzymes, said method comprising:
applying an enzyme-based cleaning composition comprising a lipase to a surface; and
applying an enzyme-inactivation composition to said surface, wherein said enzyme-inactivation composition comprises a sulfonated peroxycarboxylic acid according to formula 1:
wherein: R1 is hydrogen, or a substituted or unsubstituted Cm alkyl group; R2 is a substituted or unsubstituted Cn alkyl group; X is a hydrogen atom, a cationic group, or an ester forming moiety; Y is a hydrogen atom, a cationic group, or an ester forming moiety; n is 1 to 10; m is 1 to 10; and n+m is less than or equal to 18,
wherein said enzyme-inactivation composition is effective for inactivation of said lipase as determined by an ELISA analysis.
2. The method of claim 1 , wherein Y is a hydrogen atom.
3. The method of claim 1 , wherein said enzyme-inactivation composition further comprises an oxidizing reagent, wherein said oxidizing reagent is not a chlorine-containing compound.
4. The method of claim 1 , wherein said enzyme-inactivation composition further comprises hydrogen peroxide.
5. The method of claim 1 , wherein said enzyme-inactivation composition further comprises a C1 to C22 carboxylic or peroxycarboxylic acid.
6. The method of claim 1 , wherein said applying an enzyme-inactivation composition to said surface is carried out at a temperature in the range of 10° C. to 70° C.
7. The method of claim 1 , wherein said enzyme-inactivation composition has a pH of 7 to 9.
8. The method of claim 1 , wherein said enzyme-inactivation composition further comprises:
10 to 20 wt-% sulfonated oleic acid;
up to 10 wt-% acetic acid;
5 to 10 wt-% peracetic acid;
1 to 5 wt-% octanoic acid;
1 to 5 wt-% peroxyoctanoic acid;
1 to 5 wt-% hydroxyethane diphosphonic acid; and
8 to 35 wt-% hydrogen peroxide.
9. The method of claim 1 , wherein said enzyme-inactivation composition of formula 1 is selected from 11-sulfo-undecanoic acid, 10,11-disulfo-undecanoic acid, sulfonated oleic acid, sulfonated linoleic acid, sulfonate palmitoleic acid, and sulfonated stearic acid.
10. The method of claim 1 , wherein said surface comprises a surface on process equipment.
11. The method of claim 10 , wherein said surface comprises a food or beverage preparation surface.
12. The method of claim 1 , wherein said surface comprises a fabric or textile.
13. The method of claim 1 , wherein said surface comprises a membrane.
14. The method of claim 1 , wherein applying said enzyme-based cleaning composition comprises a clean-in-place procedure.
15. The method of claim 1 , further comprising rinsing said surface after applying the enzyme-based cleaning composition to said surface.
16. The method of claim 1 , wherein said enzyme-inactivation composition is effective for inactivating enzymes at room temperature.
17. The method of claim 1 , wherein said enzyme-inactivation composition is applied to said surface at room temperature.