Self-contained biological analysis
Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.
1. A device for analyzing a sample, comprising:
a first support,
a second support,
an opening between the first and second supports configured to receive a flexible container, the container comprising a plurality of blisters fluidly connected by a plurality of channels and a second-stage chamber fluidly connected to the blisters, the plurality of blisters including a first-stage blister,
a plurality of actuators provided on at least one of the first or second supports, each of the plurality of actuators positioned corresponding to various blisters and channels of the container, each actuator configured to apply pressure to the corresponding blister or channel, wherein sufficient pressure on one of the plurality of blisters will force the sample from that blister,
a plurality of seals, each seal provided on at least one of the first or second supports, wherein sufficient pressure by the seals on all channels connected to one of the plurality of blisters will contain the sample within that blister,
a first heating/cooling device provided on one of the first or second supports, the first heating/cooling device configured for thermal cycling contents of the first-stage blister, a plurality of seals, each seal provided on at least one of the first or second supports, wherein sufficient pressure by the seals on all channels connected to one of the plurality of blisters will contain the sample within that blister, and
a second heating/cooling device provided on one of the first or second supports, the second heating/cooling device configured for thermal cycling contents of the second-stage chamber.
2. The device of claim 1 , wherein the second-stage chamber comprises a plurality of second-stage amplification blisters and the second heating/cooling device is configured for thermal cycling the plurality of second-stage amplification blisters.
3. The device of claim 2 , further comprising optics mounted in the device and positioned to detect fluorescence from the plurality of second-stage amplification blisters.
4. The device of claim 1 , further comprising a magnet retractably mounted on one of the first or second supports and positioned adjacent to one of the blisters.
5. The device of claim 1 , further comprising a computer for controlling the actuators and the first and second heating/cooling devices.
6. The device of claim 1 , further comprising a feedback mechanism positioned to sense whether the sample has been moved into one of the blisters.
7. The system of claim 1 , wherein the actuators are pneumatic bladders.
8. A device for analyzing a sample, comprising:
a first support,
a second support,
an opening between the first support and the second support, the opening configured to receive a flexible container, the flexible container comprising:
i. a plurality of fluidly connected reaction blisters including an amplification blister, wherein the plurality of reaction blisters is connected via a plurality of channels,
ii. an additional set of second-stage amplification chambers fluidly connected to the amplification blister, each additional second-stage amplification chamber configured for further amplification of a specific nucleic acid that may be present in the sample, and
iii. one or more sealable ports fluidly connected to the plurality of fluidly connected reaction blisters,
a plurality of actuators, each actuator provided on at least one of the first support or second support, each actuator positioned corresponding to individual blisters of the container, wherein sufficient pressure on one of the plurality of blisters will force the sample from the blister,
a plurality of seals, each seal provided on at least one of the first or second supports, wherein sufficient pressure by the seals on all channels connected to one of the plurality of blisters will contain the sample within that blister,
a first heating/cooling device fixedly mounted on the first support, the first heating/cooling device positioned for thermal cycling contents of the first-stage blister, and
a second heating/cooling device fixedly mounted on the first support, the second heating device positioned for thermal cycling contents of the second-stage chamber after the first heating/cooling device thermal cycles the contents of the first-stage blister.
9. The device of claim 8 , further comprising a plurality of seals, each seal provided on at least one of the first support or second support, the seals positioned to apply pressure to a channel, wherein sufficient pressure by the seals on all channels connected to one of the plurality of blisters will contain the sample within that blister.
10. The device of claim 8 , wherein each of the seals comprises an actuator mounted on at least one of the first support or second support.
11. The device of claim 10 , wherein the actuators are pneumatic bladders.