Methods for improving crop yield
Compositions and methods for increasing plant growth and yield are provided. Compositions comprise the high yield gene TEL, promoters and enhancers to increase the expression of a TEL gene in a plant of interest. By enhancing the expression of at least one TEL gene in a plant, an improvement in plant growth and yield is achieved, resulting in an increase in crop yield in a field planted with such plants. A plant of interest may be transformed with a DNA construct comprising a promoter that is capable of driving expression in the plant operably linked to a coding sequence for a TEL gene. The DNA construct may comprise at least on enhancer that acts to increase expression of the TEL coding sequence. A promoter or enhancer can be inserted into the genome of the plant of interest at a site that increases the expression of the endogenous TEL coding sequence in the plant.
1. A method for increasing seed yield in a monocot plant, said method comprising:
(a) transforming monocot plants with a DNA construct comprising a heterologous promoter that drives expression in a monocot plant operably linked to a Terminal Earl-Like (TEL) nucleotide sequence, wherein said TEL nucleotide sequence encodes a protein comprising an amino acid sequence having at least 95% amino acid sequence identity to the SEQ ID NO: 2; and
(b) selecting a monocot plant obtained from step (a) transformed with said DNA construct which overexpresses said protein, and exhibits increased seed yield in the selected transgenic monocot plant as compared to a wild type monocot plant of the same species which is grown under the same growth conditions,
wherein expression of said TEL nucleotide sequence in said selected monocot plant increases the production of TEL mRNA by 5-fold to 50-fold when compared to a control monocot plant of the same monocot plant species lacking said DNA construct and grown under the same conditions, wherein said selected transformed monocot plant is rice or corn, wherein said selected transformed rice or corn plant exhibits seed yield of at least 5% to 30% more than a control rice or corn plant lacking said DNA construct and grown under the same conditions.
2. The method of claim 1 , wherein said DNA construct further comprises at least one enhancer that enhances expression of a gene in a monocot plant, and wherein said enhancer is operably linked to said heterologous promoter and said TEL nucleotide sequence.
3. The method of claim 2 , wherein said at least one enhancer is a 35S enhancer from cauliflower mosaic virus (CaMV).
4. The method of claim 2 , wherein said heterologous promoter is a TEL promoter.
5. The method of claim 2 , wherein said TEL nucleotide sequence encodes a protein comprising an amino acid sequence that has at least 97% amino acid sequence identity with the SEQ ID NO: 2.