Method for producing ciliary marginal zone-like structure
The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.
1. A method for formation of a ciliary marginal zone-like structure in a retinal tissue derived from a cell aggregate comprising
(a) preparing a cell aggregate from a pluripotent stem cell,
(b) culturing the cell aggregate to form a retinal tissue comprising at least 20% of cells positive for Chx 10,
(c) culturing the retinal tissue comprising at least 20% of cells positive for Chx10 in a serum-free medium containing a substance that enhances signal transduction mediated by Wnt for a period before the appearance of RPE65 expressing cells, thereby obtaining retinal tissue having a decreased proportion of Chx10 positive cells that is about 3% or less of the tissue,
(d) culturing the retinal tissue with the decreased proportion of Chx10 positive cells of step (c) without the substance that enhances signal transduction mediated by Wnt in a serum-free medium containing a serum replacement or a serum-containing medium to induce the formation of a ciliary marginal zone-like structure in the retinal tissue, and
(e) obtaining the retinal tissue having the ciliary marginal zone-like structure, wherein the ciliary marginal zone-like structure comprises Rdh10 positive cells.
2. The method according to claim 1 , wherein the decreased proportion of Chx10 positive cells in step (c) is within 3% to 1% of the tissue.
3. The method according to claim 2 , wherein the culturing in step (d) is until the proportion of Chx10 positive cells present in the retinal tissue reaches 50% or more of the tissue.
4. The method according to claim 1 , wherein the pluripotent stem cell is a human pluripotent stem cell.
5. The method according to claim 1 , wherein the culturing of the retinal tissue with the decreased proportion of Chx10 positive cells in step (d) is in a serum-free medium containing a serum replacement.
6. The method according to claim 1 , wherein the culturing of the retinal tissue with the decreased proportion of Chx10 positive cells in step (d) is in a serum-containing medium.
7. The method according to claim 1 , wherein the decreased proportion of Chx10 positive cells in step (c) is about 3% of the tissue.
8. The method according to claim 1 , wherein the culturing of the cell aggregate in step (b) is to form a retinal tissue comprising at least 40% of cells positive for Chx10, and the culturing in step (c) is culturing the retinal tissue comprising at least 40% of cells positive for Chx10.
9. The method according to claim 1 , wherein the culturing of the retinal tissue in step (c) is for 2 to 5 days.
10. The method according to claim 9 , wherein the culturing of the retinal tissue in step (c) is for 2 to 3 days.
11. The method according to claim 1 , wherein the culturing of the cell aggregate in step (b) is to form a retinal tissue comprising at least 40% of cells positive for Chx10, and the culturing in step (c) is culturing the retinal tissue comprising at least 40% of cells positive for Chx10 for 2 to 5 days.
12. A method for producing a neural retina comprising
(a) preparing a cell aggregate from a pluripotent stem cell,
(b) culturing the cell aggregate to form a retinal tissue comprising at least 20% of cells positive for Chx10,
(c) culturing the retinal tissue comprising at least 20% of cells positive for Chx10 in a serum-free medium containing a substance that enhances signal transduction mediated by Wnt for a period before appearance of RPE65 expressing cells, thereby obtaining retinal tissue having a decreased proportion of Chx10 positive cells that is about 3% or less of the tissue,
(d) culturing the retinal tissue with the decreased proportion of Chx10 positive cells of step (c) without the substance that enhances signal transduction mediated by Wnt in serum-free medium containing a serum replacement or a serum-containing medium to induce formation of a ciliary marginal zone-like structure in the retinal tissue,
(e) obtaining the retinal tissue having the ciliary marginal zone-like structure, wherein the ciliary marginal zone-like structure comprises Rdh10 positive cells, and a retinal pigment epithelium and a neural retina are present adjacent to the ciliary marginal zone-like structure, and
(f) cutting out the neural retina from the retinal tissue in step (e), thereby obtaining a purified neural retina.
13. The method according to claim 12 , wherein the culturing of the cell aggregate in step (b) is to form a retinal tissue comprising at least 40% of cells positive for Chx10, and the culturing in step (c) is culturing the retinal tissue comprising at least 40% of cells positive for Chx10.
14. The method according to claim 12 , wherein the culturing of the retinal tissue in step (c) is for 2 to 5 days.
15. The method according to claim 12 , wherein the culturing of the cell aggregate in step (b) is to form a retinal tissue comprising at least 40% of cells positive for Chx10, and the culturing in step (c) is culturing the retinal tissue comprising at least 40% of cells positive for Chx10 for 2 to 5 days.