Means and methods for influencing the stability of cells
View Patent ↗The invention provides a method for influencing the stability of an antibody producing cell, comprising directly or indirectly influencing the amount of BCL6 and/or Blimp 1 expression product within said antibody producing cell. Stable antibody producing cells and cell lines are also provided, as well as methods for producing antibodies using such cells and/or cell lines.
1. A composition comprising: an isolated antibody producing cell that is stable for at least nine weeks, comprising an exogenous nucleic acid sequence encoding BCL6 or a functional part thereof; and IL-21 and/or IL-10.
2. The composition of claim 1 , comprising IL-21.
3. The composition of claim 1 , comprising an exogenous nucleic acid sequence encoding constitutively active BCL6.
4. The composition of claim 1 , wherein said cell is a human cell producing human antibodies.
5. The composition of claim 1 , wherein the antibody producing cell produces an antibody of interest.
6. A composition comprising: an isolated antibody producing cell that is stable for at least nine weeks, comprising an exogenous nucleic acid sequence encoding BCL6 or a functional part thereof; and IL-21.
7. An isolated antibody producing cell that is stable for at least nine weeks, comprising (i) an exogenous nucleic acid sequence encoding BCL6 or a functional part thereof; and (ii) IL-21.