Methods for treating pain by topical application and transdermal delivery of botulinum toxin
Improved formulations for transdermal delivery of botulinum toxin are disclosed. The formulations include, for example, botulinum toxin non-covalently associated with a positively charged backbone having branching or efficiency groups. The formulations also include a partitioning agent, oligo-bridge, or polyanion bridge, and may optionally contain a viscosity modifying agent. The formulations are designed for topical application onto the skin of a patient and may be used to treat wrinkles, hyperhidrosis, and other health-related problems. Kits for administration are also described.
1. A method of treating pain in a patient in need thereof, said method comprising:
topically administering to an affected area of the patient in need of treatment, a pharmaceutically acceptable composition comprising:
botulinum toxin,
a carrier comprising a positively charged polymeric backbone with positively charged efficiency groups covalently attached thereto;
a poloxamer; and
a dermatologically or pharmaceutically acceptable excipient, diluent, or medium;
wherein the botulinum toxin is non-covalently associated with the positively charged polymeric backbone; and wherein the carrier comprising the positively charged backbone is the sole necessary agent for non-covalently associating with the botulinum toxin and delivering the botulinum toxin to the affected area so as to treat the s pain in said patient.
2. The method according to claim 1 , wherein the pain is selected from one or more of neurologic pain, migraine headache pain, non-migraine headache pain, or muscle-spasm pain.
3. The method according to claim 2 , wherein the pain is neurologic pain.
4. The method according to claim 2 , wherein the pain is migraine headache pain.
5. The method according to claim 2 , wherein the pain is non-migraine headache pain.
6. The method according to claim 2 , wherein the pain is muscle-spasm pain.
7. The method according to claim 1 , wherein the composition is topically administered a patient's skin.
8. The method according to claim 1 or claim 2 , wherein the positively charged polymeric backbone is polylysine or polyethyleneimine (PEI).
9. The method according to claim 8 , wherein the positively charged polymeric backbone has a molecular weight of less than 21,000.
10. The method according to claim 8 , wherein the positively charged efficiency groups are either protected oligoarginine or TAT domains.
11. The method according to claim 10 , wherein the composition further comprises a viscosity modifying agent or a gelling agent.
12. The method according to claim 11 , wherein the viscosity modifying agent or gelling agent is polyethylene glycol (PEG) or hydroxypropylcellulose (HPC).
13. The method according to claim 10 , wherein the positively charged efficiency groups are amino acid sequences selected from the group consisting of (gly)p-RGRDDRRQRRR-(gly)q (SEQ ID NO: 2), (gly)p-YGRKKRRQRRR-(gly)q (SEQ ID NO: 3), and (gly)p-RKKRRQRRR-(gly)q (SEQ ID NO: 4), wherein the subscripts p and q are each independently an integer of from 0 to 20.
14. The method according to claim 13 , wherein the positively charged efficiency groups include the amino acid sequence (gly)p-RGRDDRRQRRR-(gly)q (SEQ ID NO: 2), wherein the subscripts p and q are each independently an integer of from 0 to 20.
15. The method according to claim 13 , wherein the positively charged efficiency groups include the amino acid sequence (gly)p-YGRKKRRQRRR-(gly)q (SEQ ID NO: 3), wherein the subscripts p and q are each independently an integer of from 0 to 20.
16. The method according to claim 13 , wherein the positively charged efficiency groups include the amino acid sequence (gly)p-RKKRRQRRR-(gly)q (SEQ ID NO: 4), wherein the subscripts p and q are each independently an integer of from 0 to 20.
17. The method according to claim 13 , wherein the subscripts p and q are each independently an integer of from 0 to 8.
18. The method according to claim 13 , wherein the subscripts p and q are each independently an integer of from 2 to 5.
19. The method according to claim 8 , wherein the positively charged polymeric backbone is polylysine and the positively charged efficiency groups include amino acid sequence (gly)p-RGRDDRRQRRR-(gly)q (SEQ ID NO: 2), wherein the subscripts p and q are each independently an integer of from 2 to 5.
20. The method according to claim 8 , wherein the positively charged polymeric backbone is polylysine and the positively charged efficiency groups include amino acid sequence (gly)p-YGRKKRRQRRR-(gly)q (SEQ ID NO: 3), wherein the subscripts p and q are each independently an integer of from 2 to 5.
21. The method according to claim 8 , wherein the positively charged polymeric backbone is polylysine and the positively charged efficiency groups include amino acid sequence (gly)p-RKKRRQRRR-(gly)q (SEQ ID NO: 4), wherein the subscripts p and q are each independently an integer of from 2 to 5.
22. The method according to 1, wherein the botulinum toxin is a serotype selected from the group consisting of serotype A, B, C, D, E, F, G and mixtures thereof.
23. The method according to claim 22 , wherein the botulinum toxin is of serotype A.
24. The method according to claim 19 , wherein the botulinum toxin is of serotype A.
25. The method according to claim 20 , wherein the botulinum toxin is of serotype A.
26. The method according to claim 21 , wherein the botulinum toxin is of serotype A.
27. The method according to claim 23 , wherein the botulinum toxin has a molecular weight of 150,000.
28. The method according to claim 24 , wherein the botulinum toxin has a molecular weight of 150,000.
29. The method according to claim 25 , wherein the botulinum toxin has a molecular weight of 150,000.
30. The method according to claim 26 , wherein the botulinum toxin has a molecular weight of 150,000.
31. The method according to claim 19 , wherein the polylysine has a molecular weight of about 21,000.
32. The method according to claim 20 , wherein the polylysine has a molecular weight of about 21,000.
33. The method according to claim 21 , wherein the polylysine has a molecular weight of about 21,000.
34. The method according to claim 11 , wherein the positively charged backbone is polylysine and the amino acid sequence is attached to the polylysine via either the C-terminus or the N-terminus of the amino acid sequence.
35. The method according to claim 19 , wherein the amino acid sequence is attached to the polylysine via either the C-terminus or the N-terminus of the amino acid sequence.
36. The method according to claim 24 , wherein the amino acid sequence is attached to the polylysine via either the C-terminus or the N-terminus of the amino acid sequence.
37. The method according to claim 25 , wherein the amino acid sequence is attached to the polylysine via either the C-terminus or the N-terminus of the amino acid sequence.
38. The method according to claim 26 , wherein the amino acid sequence is attached to the polylysine via either the C-terminus or the N-terminus of the amino acid sequence.
39. The method according to claim 10 , wherein the positively charged efficiency groups comprise at least about 0.05% by weight of the total carrier weight in the composition.
40. The method according to claim 39 , wherein the positively charged efficiency groups comprise from about 0.1% to about 30%% by weight of the total carrier weight in the composition.
41. The method according to claim 13 , wherein the composition comprises botulinum toxin of serotype A, polylysine having covalently attached thereto positively charged efficiency groups having the amino acid sequence (gly)p-RKKRRQRRR-(gly)q (SEQ ID NO. 4), wherein the subscripts p and q are each independently an integer of from 0 to 20, and poloxamer.
42. The method according to claim 41 , wherein the positively charged efficiency groups comprise from about 0.1% to about 30%% by weight of the total carrier weight in the composition.
43. The method according to claim 41 , wherein the polylysine has a molecular weight of about 21,000.
44. The method according to claim 41 , wherein the botulinum toxin of serotype A has a molecular weight of 150,000.
45. The method according to claim 1 , wherein the dermatologically or pharmaceutically acceptable excipient, diluent, or medium is an aqueous diluent comprising a saline solution of 15% poloxamer, and wherein the carrier and the botulinum toxin are present in the saline solution in a ratio corresponding to 0.5 μg carrier to 100 U of toxin.