Organ chips and uses thereof
Disclosed herein are organ chips that can be individually used or integrated together to form different microphysiological systems, e.g., for use in cell culturing, drug screening, toxicity assays, personalized therapeutic treatment, scaffolding in tissue repair and/or replacement, and/or pharmacokinetic or pharmacodynamics studies.
1. A method comprising:
(a) contacting muscle cells, organ-specific parenchymal cells that are a different cell type from the muscle cells, or a combination thereof, with at least one active agent for a period of time,
wherein the organ-specific parenchymal cells and the muscle cells are cultured independently in at least two different organ chips, wherein said at least two different organ chips are each independently configured according to either a first organ chip or a second organ chip, and are coupled by at least one connecting means between said at least two different organ chips,
wherein at least a first type of the organ-specific parenchymal cells are cultured on a first side of an at least partially porous first membrane disposed in a body of the first organ chip, the first organ chip comprising: the body comprising a central channel therein, and the first membrane positioned within the central channel and along a plane, wherein the first membrane is configured to separate the central channel to form two sub-channels, and a second side of the first membrane is seeded with vascular endothelial cells, and
wherein the muscle cells are cultured in the second organ chip comprising: a body comprising a chamber enclosing a plurality of muscular thin films that include the muscle cells adhered to a biopolymer substrate, the muscular thin films configured such that contraction of the adhered muscle cells causes the muscular thin films to bend, wherein the first chamber is configured to measure contraction of the adhered muscle cells; and
(b) measuring a response of the cells cultured in said at least two different organ chips to determine the effect of the at least one active agent on at least two organs, the measuring including measuring a response by the contraction of the adhered muscle cells.
2. The method of claim 1 , wherein the cells are contacted with said at least one active agent by flowing the active agent in a liquid through the channel or chamber where the cells are cultured.
3. The method of claim 2 , wherein the active agent is added to a liquid flowing through the channel or the chamber.
4. The method of claim 3 , wherein the liquid comprises cell culture medium, blood, or a combination thereof.
5. The method of claim 2 , wherein the active agent is in a form of an aerosol flowing through the channel or the chamber.
6. The method of claim 1 , wherein the response of the cells is selected from the group consisting of viability, proliferation, respiration, metabolism, cell migration, cell contractility, differential expression of cell biomarkers, production and/or release of biomolecules, action potentials, and any combinations thereof.
7. The method of claim 1 , wherein the organ-specific parenchymal cells, the muscle cells, or both are collected from a subject or differentiated from stem cells collected from the subject.