Eluting analytes from bead arrays
View Patent ↗Microarray compositions suitable for analysis by one or several spectrographic methods are disclosed. In an embodiment, a microarray composition includes a three-dimensional solid support and a plurality of reactive microbeads positioned on the solid support in spatially distinct and addressable locations.
1. A method of performing elution of an analyte from a bead array, the method comprising the steps of:
providing a bead array, the bead array comprising a solid support, a bead and an analyte associated with the bead, wherein the solid support comprises a microwell and the bead is submerged in the microwell; and
causing migration of the analyte from the bead onto at least one of a bottom surface of the microwell, a sidewall of the microwell, and a surface outside the microwell that is adjacent to the microwell, wherein the causing migration step comprises exposing the solid support to an aerosol.
2. The method of claim 1 wherein a diameter of the microwell is less than 1 mm.
3. The method of claim 1 wherein a diameter of the microwell exceeds a diameter of the bead by at least 10 microns.
4. The method of claim 1 further comprising a step of delivering a layer of MALDI matrix to the bottom surface of the microwell.
5. The method of claim 1 wherein the analyte is selected from a group consisting of peptides, peptoids, peptidomimetics, proteins, antibodies, metabolites, lipids, carbohydrates, nucleic acids and drug compounds.
6. The method of claim 1 wherein the analyte comprises two chemically distinct peptides.
7. The method of claim 1 further comprising the step of reducing dimensions of the bead while the bead remains disposed in the microwell.
8. The method of claim 1 further comprising the step of removing the bead from the solid support.
9. The method of claim 8 wherein the bead removing step comprises exposing the bead to a stream of gas.
10. The method of claim 1 wherein the sidewall of the microwell is hydrophobic.
11. The method of claim 1 wherein the sidewall of the microwell comprises a polymer.
12. The method of claim 1 wherein the sidewall of the microwell comprises silicone.
13. The method of claim 1 further comprising the step of depositing a layer of MALDI matrix onto the solid support.
14. A method of performing elution of an analyte from a bead array, the method comprising the steps of:
providing a bead array, the bead array comprising a solid support, a bead and an analyte associated with the bead, wherein the solid support comprises a microwell and the bead is submerged in the microwell, wherein the analyte comprises two chemically distinct peptides, and wherein the two chemically distinct peptides are selected from the group consisting of peptides with different isotope abundance, peptides with different fluorescent labels, peptides with at least one amino acid substitution in an otherwise identical amino acid sequence and peptides with a different number or different positions of post-translational modifications (PTMs) in an otherwise identical amino acid sequence; and
causing migration of the analyte from the bead onto at least one of a bottom surface of the microwell, a sidewall of the microwell, and a surface outside the microwell that is adjacent to the microwell.
15. A method of performing elution of an analyte from a bead array, the method comprising the steps of:
providing a bead array, the bead array comprising a solid support, a bead and an analyte associated with the bead, wherein the solid support comprises a microwell and the bead is submerged in the microwell,
causing migration of the analyte from the bead onto at least one of a bottom surface of the microwell, a sidewall of the microwell, and a surface outside the microwell that is adjacent to the microwell, and
repositioning the analyte on the solid support, the repositioning step being performed after the causing migration step and comprising dissolving and subsequently precipitating the analyte on the solid support whereby localization of the analyte on the solid support changes.