Method for detecting microorganism having fluorescent characteristics with respect to specific wavelength
A method for determining a number of individuals of microorganisms included in a sample using an optical apparatus, comprising steps of: collecting, by the image acquisition unit, light radiated by microorganisms which radiate only light of a specific wavelength range and are included in the sample; generating, by the image acquisition unit, an image of the collected image; displaying, by the image processing unit, the image generated by the image acquisition unit on a screen, analyzing each of pixels forming the screen on which the image has been displayed, and classifying a plurality of pixels which belongs to the pixels included in the screen and continuously emit light to be a single object; and calculating, by the image processing unit, a total number of objects classified in the screen.
1. A method for determining a number of individuals of microorganisms included in a sample using an optical apparatus, comprising a light source unit for supplying light to a sample comprising microorganisms which reflect only light of a specific wavelength range and have a fluorescent characteristic and a detection unit comprising an image acquisition unit for generating an image by collecting the light reflected by the microorganisms and an image processing unit for processing the generated image, the method comprising steps of:
(a) collecting, by the image acquisition unit, light radiated by microorganisms which radiate only light of a specific wavelength range and are included in the sample;
(b) generating, by the image acquisition unit, an image of the collected image;
(c) displaying, by the image processing unit, the image generated by the image acquisition unit on a screen, analyzing each of pixels forming the screen on which the image has been displayed, classifying a plurality of pixels which belongs to the pixels included in the screen and continuously emit light as a single object, and extracting only an object that belongs to said single object and has an average brightness value of the pixels forming the object, corresponding to a predetermined brightness range, as an active object; and wherein in the step (c), the image processing unit classifies the plurality of pixels continuously emitting light to be the single object, wherein the image processing unit determines only a plurality of pixels which continuously emits light within a range of a predetermined contrast range along with one or more of all of neighboring pixels with respect to the pixels forming the screen to be the single object;
(d) calculating, by the image processing unit, a total number of active objects classified in the screen.
2. The method of claim 1 ,
wherein in the step (c), the image processing unit performs edge detection processing on the generated image.
3. The method of claim 1 , wherein:
in the step (c), the image processing unit classifies only an object belonging to the objects and having the number of pixels forming the single object within a range of a predetermined number to be a target object, and
in the step (d), the total number of objects included in the screen and calculated by the image processing unit is a total number of target objects.