Biosensor comprising metal nanoparticles
The present invention discloses a biosensor for visual detection of an analyte, based on the light to heat conversion properties of metal nanoparticles: the analyte is visually detected by the color change in the support areas (where the analyte is present), produced as a result of the heat generated by the metal nanoparticles where they are irradiated with an external light source. Use of said biosensor in a method for the detection of analytes is also claimed.
1. A method for detecting an analyte in a sample, comprising the steps of:
(a1) adding the sample comprising the analyte to a support with a first analyte recognition molecule immobilized thereon, wherein the support comprises a heat sensitive surface;
(b1) incubating the support of step (a1) with metal nanoparticles functionalized with a second analyte recognition molecule; and
(c1) irradiating the support of step (b1) with an external light source; or
(a2) adding the sample comprising the analyte to a support with a first analyte recognition molecule immobilized thereon, wherein the support does not comprises a heat sensitive surface;
(b2) incubating the support of step (a2) with metal nanoparticles functionalized with a second analyte recognition molecule;
(c2) placing the support of step (b2) on a heat sensitive surface; and
(d2) irradiating the support of step (c2) with an external light source.
2. The method of claim 1 , wherein the analyte is an additive, a drug, a pathogenic microorganism, a food component, a pesticide, a toxic compound or oxygen.
3. The method of claim 1 , wherein the analyte is visually detected by a color change in the support area where the analyte is present; and wherein the color change is produced as a result of heat generated by the metal nanoparticles when they are irradiated with the external light source.
4. The method of claim 1 , wherein the heat sensitive surface comprises any surface capable of experiencing a structural change when heated, resulting in image development.
5. The method of claim 1 , wherein the external light source is capable of causing excitation of surface plasmon absorption band of the metal nanoparticles.
6. The method of claim 4 , wherein the external light source produces radiation of wavelength of between 750 nm and 110 nm.
7. The method of claim 4 , wherein the external light source is a monochromatic or a polychromatic light source.
8. The method of claim 1 , wherein the metal nanoparticles comprise gold, silver or copper.
9. The method of claim 1 , wherein the heat sensitive surface is a thermal paper or a temperature sensitive polymer.
10. The method of claim 9 , wherein the temperature sensitive polymer comprises poly(N-isopropylacrylamide), poly(N-vinylpiperidine) or poly(N-inylcaprolactam).
11. A method for detecting an analyte in a sample, comprising the steps of:
(a3) adding the sample comprising the analyte to a support with a first analyte recognition molecule immobilized thereon, wherein the support comprises a heat sensitive surface;
(b3) incubating the support of step (a3) with a second analyte recognition molecule bound to at least one label molecule;
(c3) incubating the support of step (b3) with metal nanoparticles functionalized with at least one molecule capable of specifically binding to the label molecule; and
(d3) irradiating the support of step (c3) with an external light source; or
(a4) adding the sample comprising the analyte to a support with a first analyte recognition molecule immobilized thereon, wherein the support does not comprise a heat sensitive surface;
(b4) incubating the support of step (a4) with a second analyte recognition molecule bound to at least one label molecule;
(c4) incubating the support of step (b4) with metal nanoparticles functionalized with at least one molecule capable of specifically binding to the label molecule;
(d4) placing the support of step (c4) on a heat sensitive surface; and
(d4). irradiating the support of step (d4) with an external light source.
12. The method of step 11 , wherein the analyte is visually detected by a color change in the support area where the analyte is present; and wherein the color change is produced as a result of heat generated by the metal nanoparticles when they are irradiated with the external light source.
13. The method of claim 11 , wherein the label molecule is biotin and wherein the at least one molecule capable of specifically binding the label molecule is avidin or streptavidin; or wherein the label molecule is avidin or streptavidin and the at least one molecule capable of specifically binding the label molecule is biotin.
14. The method of claim 11 , wherein the heat sensitive surface comprises any surface capable of experiencing a structural change when heated, resulting in image development.
15. The method of claim 11 , wherein the external light source is capable of causing excitation of surface plasmon absorption band of the metal nanoparticles.
16. The method of claim 14 , wherein the external light source produces radiation of wavelength of between 750 nm and 110 nm.
17. The method of claim 14 , wherein the external light source is a monochromatic or a polychromatic light source.
18. The method of claim 11 , wherein the metal nanoparticles comprise gold, silver or copper.
19. The method of claim 11 , wherein the heat sensitive surface is a thermal paper or a temperature sensitive polymer.
20. The method of claim 19 , wherein the temperature sensitive polymer comprises poly(N-isopropylacrylamide), poly(N-vinylpiperidine) or poly(N-vinylcaprolactam).
21. The method of claim 11 , wherein the analyte is an additive, a drug, a pathogenic microorganism, a food component, a pesticide, a toxic compound or oxygen.