PtDUF266 gene regulating cell wall biosynthesis and recalcitrance in populus
This disclosure provides genetically modified plants having desirable levels of sugar release, cellulose content and reduction of recalcitrance; methods of genetically modifying plants to modulate sugar release, cellulose and lignin contents; and uses of such plants. The inventors have determined that genetic modification of PdDUF266A from Populus , encoded by locus Potri.011G009500 resulted in transgenic Populus trees with changes in lignin and cellulose content as well as altered sugar release phenotypes. Plants with altered sugar release, cellulose and lignin content, based on modulation of the expression or activity of the PdDUF266A gene, have diverse uses including pulp and paper production, and biofuel and bioproducts production.
1. A method for increasing biomass, cellulose content or sugar release in a Populus plant, the method comprising:
(i) transforming Populus plants with a recombinant binary vector comprising a DNA expression construct, wherein the DNA expression construct comprises a promoter operably linked to the exogenous coding sequence consisting the nucleic acid sequence as set forth in SEQ ID NO: 37 encoding the protein of SEQ ID NO: 19;
(ii) expressing said DNA construct in said Populus plants;
wherein expression of said protein of SEQ ID NO: 19 is increased in said Populus plants; and
(iii) selecting a transformed Populus plant from said transformed Populus plants of step (ii) which over-expresses said protein of SEQ ID NO: 19 and wherein said selected transformed Populus plant exhibits increase in biomass, cellulose content or sugar release as compared to a wild-type untransformed Populus plant of the same species lacking said DNA expression construct.
2. The method according to claim 1 , wherein said biomass is increased.
3. The method according to claim 1 , wherein said cellulose content is increased.
4. The method according to claim 1 , wherein said sugar release is increased.
5. The method according to claim 4 , wherein the sugar is glucose.
6. The method according to claim 4 , wherein the sugar is xylose.
7. A method for decreasing lignin content in a Populus plant, the method comprising:
(i) transforming Populus plants with a recombinant binary vector comprising a DNA expression construct, wherein the DNA expression construct comprises a promoter operably linked to the exogenous coding sequence consisting the nucleic acid sequence as set forth in SEQ ID NO: 37 encoding the protein of SEQ ID NO: 19;
(ii) expressing said DNA construct in said Populus plants; wherein expression of said protein of SEQ ID NO: 19 is increased in said Populus plants; and
(iii) selecting a transformed Populus plant from said transformed Populus plants of step (ii) which over-expresses said protein of SEQ ID NO: 19 and wherein said selected transformed Populus plant exhibits decrease in lignin content as compared to a wild-type untransformed Populus plant of the same species lacking said DNA expression construct.