Method and apparatus for preparing a cellular container comprising means for pre-analysis of a sample that has been taken
A method for preparing a cell suspension for analysis includes at least: taking a sample of the cell suspension using a pipetting-dispensing device, which includes at least one pipe, and depositing the sample present in the pipetting-dispensing device in an analysis container. Between the taking of the sample and the depositing, the method includes at least one pre-analysis of the sample, which is carried out by a pre-analysis device placed on the pipe of the pipetting-dispensing device.
1. A method for preparing a cell suspension for analysis, comprising at least the following successive steps:
(a) taking a first portion of a sample of the cell suspension using a pipettor, said pipettor comprising at least one pipe; and
(b) pre-analyzing the entire first portion of the sample that has been taken by a pre-analysis analyzer placed on the pipe of the pipettor, said pre-analyzing comprising measuring the cell density of the first portion of the sample that has been taken and comparing the measured cell density to a reference density,
wherein,
(i) if the measured cell density is lower than the reference density, the pre-analyzing further comprises taking a second portion of the sample of the cell suspension using the pipettor so that a combined volume of the first portion and the second portion in the pipettor has a volume of the cell suspension that is larger than a standard volume, or
(ii) if the measured cell density is higher than the reference density, the pipettor is used to take a volume of the first portion of the sample of the cell suspension, wherein the volume is smaller than the standard volume, so as to obtain deposit smears with a homogenous density, and
(c) depositing the sample present in the pipettor in an analysis container,
wherein steps (a)-(c) are repeated to provide a number of deposited smears of homogenous density for analysis.
2. The method according to claim 1 , further comprising:
establishing the volume that has been taken.
3. The method according to claim 1 , wherein the cell density is measured by emitting light in the pipe through the sample and measuring the attenuation or diffraction of light having crossed through the sample.
4. The method according to claim 1 , wherein the pipettor comprises at least one needle suitable for taking a sample of the suspension, the pipe extending between at least one of the at least one needle and a suction-discharger, wherein in step (a), at least part of the sample that has been taken passes through the pipe across from the pre-analysis analyzer.