IP Library Granted Patent US 10,244,765
Granted Patent B2
US 10,244,765 · App. 15/984,795 · Granted Apr 2, 2019

Inhibiting or reducing fungal growth

Inventors: George E. Pierce (Canton, GA); Sidney Crow (Smyrna, GA)
Assignee: Georgia State University Research Foundation, Inc.
A01N63/02A01N63/00C12N1/20C12Y305/05C12Y401/99C12Y402/01084C12Y404/01009
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Quick Facts
Patent No.
US 10,244,765
App. No.
15/984,795
Granted
Apr 2, 2019
Kind
B2
Abstract

Provided are methods and compositions for inhibiting or reducing fungal growth. The methods comprise exposing a location to a composition comprising one or more enzymes, one or more bacteria, and/or an enzymatic extract, wherein the one or more enzymes, one or more bacteria, and/or the enzymatic extract isolated from one or more bacteria are exposed to location in a quantity sufficient to inhibit or reduce fungal growth.

Claims (30)

1. A method for inhibiting or reducing fungal growth in grain, comprising exposing the grain to a composition comprising one or more bacteria, wherein the one or more bacteria are selected from the group consisting of Rhodococcus rhodochrous DAP 96253, Rhodococcus rhodochrous DAP 96622, Rhodococcus erythropolis , and combinations thereof, and wherein the one or more bacteria are provided in a quantity sufficient to inhibit or reduce fungal growth on the grain.

2. The method of claim 1 , wherein the one or more bacteria are induced to produce one or more enzymes selected from the group consisting of nitrile hydratases, amidases, asparaginases, ACC deaminases, cyanoalanine synthase-like enzymes, monooxygenases, dioxygenases, cyanidases, and combinations thereof.

3. The method of claim 2 , wherein the composition further comprises the one or more enzymes or an enzymatic extract produced by the one or more bacteria.

4. The method of claim 1 , wherein the composition further comprises an inducing agent selected from the group consisting of urea, methyl carbamate, methacrylamide, acetamide, cobalt, asparagine or asparagine derivative, and combinations thereof.

5. The method of claim 1 , wherein the composition further comprises a stabilizing agent.

6. The method of claim 5 , wherein the stabilizing agent is trehalose.

7. The method of claim 1 , wherein the one or more bacteria are fixed with glutaraldehyde and cross-linked.

8. The method of claim 1 , wherein the one or more bacteria are provided in a coating layer.

9. The method of claim 8 , wherein the coating layer is selected from a hydrophobic fatty acid polyester coating or a wax.

10. The method of claim 1 , wherein the composition further comprises a magnetic material.

11. The method of claim 8 , wherein the magnetic material comprises iron.

12. The method of claim 1 , wherein the composition is administered in the proximity of, but not directly on, the grain.

13. The method of claim 1 , wherein the composition is administered directly on the grain.

14. The method of claim 1 , wherein the grain is in a grain silo.

15. The method claim 1 , wherein the grain is selected from the group consisting of a cereal grain, a starchy grain, a grain legume, and an oilseed.

16. The method of claim 15 , wherein the cereal grain is selected from the group consisting of maize, sorghum, fonio, millet, rice, rye, barley, oat, triticale, wild rice, and teff.

17. The method of claim 15 , wherein the starchy grain is selected from the group consisting of amaranth, quinoa and buckwheat.

18. The method of claim 15 , wherein the grain legume is selected from the group consisting of soybean, common bean, chickpea, lima bean, runner bean, pigeon pea, lentil, field pea or garden pea, lupin, mung bean, fava bean, and peanut.

19. The method of claim 15 , wherein the oilseed is selected from the group consisting of rapeseed, canola, India mustard, black mustard, sunflower seed, safflower, flax seed hemp seed, and poppyseed.

20. The method of claim 1 , wherein the fungal growth is produced by a fungus selected from the group consisting of mold, yeast, mildew, fungi that cause smut, fungi that cause rust, fungi that cause diseases of plants, and fungi that cause diseases of animals.

21. The method of claim 20 , wherein the fungus is selected from the group consisting of Trichoderma species, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatis, Alternaria alternata, Epicoccum nigrum, Pichia pastoris, Geomyces destructans, Geomyces asperulatus , and Geomyces pannorum.

22. The method of claim 1 , wherein the composition is provided in liquid form.

23. The method of claim 22 , wherein the composition further comprises a liquid carrier.

24. The method of claim 23 , wherein the liquid carrier is selected from the group consisting of an aromatic hydrocarbon, a substituted naphthalene, a phthalic acid ester, an aliphatic hydrocarbon, an alcohol, and a glycol.

25. The method of claim 1 , wherein the composition is provided as a solid and the solid is applied at or near the location.

26. The method of claim 25 , wherein the solid further comprises a solid carrier.

27. The method of claim 26 , wherein the solid carrier is selected from the group consisting of a dust, a wettable powder, a water dispersible granule, and a mineral filler.

28. The method of claim 27 , wherein the mineral filler is selected from the group consisting of calcites, silicas, talcs, kaolins, montmorillonites, attapulgites, and mixtures thereof.

29. The method of claim 1 , wherein the composition further comprises one or more exogenous enzymes, wherein the one or more exogenous enzymes are exposed to the grain in a quantity sufficient to inhibit or reduce fungal growth.

30. The method of claim 29 , wherein the one or more exogenous enzymes are selected from the group consisting of nitrile hydratase, amidase, asparaginase, ACC deaminase, cyanoalanine synthase-like enzyme, alkane monooxygenase, ammonium monooxygenase, methane monooxygenase, toluene dioxygenase, cyanidase, and combination thereof.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 13, 2020
From: GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION
To: CROWPIERCE TECHNOLOGIES, LLC
Reel/Frame 052379/0436 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 13, 2018
From: PIERCE, GEORGE E.; CROW, SIDNEY
To: GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
Reel/Frame 046343/0696 →
Continuity (4)
Division 14773867
Provisional Application 61783395 · Mar 14, 2013
Provisional Application 61783573 · Mar 14, 2013
Related Publication 20180332859A1 · Nov 22, 2018
Cited By (3)
US 12,351,532 US 12,351,533 US 12,630,484