Cerebrospinal fluid treatment
Provided, among other things, is a method of treating cerebral spinal fluid comprising: (a) utilizing a channel of a pump to draw CSF from a subject's subarachnoid space of the subject's brain or spinal column; (b) subjecting the drawn CSF to membrane dialysis against a dialysate fluid to reduce immune or inflammatory mediators; and (c) utilizing a second channel of the synchronized dual channel pump to return the dialyzed CSF to the subarachnoid space of the subject's brain or spinal column. For example, the pump can be a synchronized dual channel pump. In one embodiment, the method further comprises: (d) removing cellular matter from the CSF to produce reduced cell content CSF, wherein the reduced cell content CSF is subjected to membrane dialysis.
1. A method of treating cerebral spinal fluid (CSF) comprising:
utilizing a channel of a synchronized dual channel pump to draw out of the body CSF from a subject's subarachnoid space of the subject's brain or spinal column;
removing cellular matter from the drawn, extracorporeal CSF to produce reduced cell content CSF;
subjecting the drawn, filtered, extracorporeal CSF to membrane dialysis against a dialysate fluid to reduce immune or inflammatory mediators; and
utilizing a second channel of the synchronized dual channel pump to return the dialyzed CSF to the subarachnoid space of the subject's brain or spinal column.
2. The method of claim 1 , wherein the reducing cellular material comprises:
flow centrifugation effective to reduce cell content;
subjecting a flow pathway for the CSF to magnetic or electrical fields that cause cells to bias towards flowing through one or more side channels;
flowing the CSF through a charged filter effective to retain human or other vertebrate cells; or
flowing the CSF through a first charged filter effective to retain human or other vertebrate cells and an oppositely charged filter effective to retain human or other vertebrate cells.
3. A method of treating or ameliorating cerebral hemorrhage comprising applying the method of claim 1 to the subject.
4. A method of treating or ameliorating acute brain injury comprising applying the method of claim 1 to the subject.
5. A method of treating or ameliorating encephalitis comprising applying the method of claim 1 to the subject.
6. A method of treating or ameliorating meningitis comprising applying the method of claim 1 to the subject.
7. A method of treating or ameliorating Alzheimer's disease comprising applying the method of claim 1 to the subject.
8. A method of treating or ameliorating Parkinson's disease comprising applying the method of claim 1 to the subject.
9. The method of claim 1 , wherein the reducing cellular material comprises flow centrifugation effective to reduce cell content.
10. The method of claim 1 , wherein the reducing cellular material comprises subjecting a flow pathway for the CSF to magnetic or electrical fields that cause cells to bias towards flowing through one or more side channels.
11. The method of claim 1 , wherein the reducing cellular material comprises flowing the CSF through a filter with pores sized to retain human or other vertebrate cells.
12. The method of claim 1 , wherein the reducing cellular material comprises flowing the CSF through a charged filter effective to retain human or other vertebrate cells.
13. The method of claim 1 , wherein the reducing cellular material comprises flowing the CSF through a first charged filter effective to retain human or other vertebrate cells and separately an oppositely charged filter effective to retain human or other vertebrate cells.