Manipulation of fluids, fluid components and reactions in microfluidic systems
Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.
1. A method of partitioning a fluid in a microfluidic network, comprising
providing a microfluidic network comprising a plurality of sections each comprising a microfluidic inlet, a first region, and a second region, wherein the plurality of sections are in fluid communication with each other;
flowing a sample fluid of volume X through a first section, wherein the sample fluid comprises a reactive component;
partitioning sample fluid at a first region of the first section into a first subset of volume of the sample fluid and a first remaining volume of the sample fluid, wherein the first subset of volume fills the first region of the first section;
flowing the first remaining volume of the sample fluid to a second section positioned downstream from the first section;
partitioning the first remaining volume of the sample fluid at a first region of the second section into a second subset of volume of the sample fluid and a second remaining volume of the sample fluid, wherein the second subset of volume fills the first region of the second section; and
sequentially partitioning the second remaining volume, and any additional remaining volumes, of the sample fluid at one or more additional sections positioned downstream from the second section into one or more additional subsets of volume of the sample fluid filling respective first regions of the one or more additional sections and corresponding one or more additional remaining volumes of the sample fluid, until the one or more additional subsets of volume of the sample fluid fails to fully fill a first region of the one or more additional sections.
2. The method of claim 1 , wherein the first subset of volume of the sample fluid is a droplet.
3. The method of claim 2 , wherein the droplet is of a first fluid bound on a solid surface of the microfluidic network.
4. The method of claim 2 , wherein flowing the sample fluid of volume X through the first section comprises flowing the sample fluid within an immiscible fluid flowing through the first section, wherein the droplet is of the sample fluid surrounded by the immiscible fluid.
5. The method of claim 4 , wherein the sample fluid is an aqueous solution.