Fluidics system for flow cytometer
A fluidics system for a flow cytometer is disclosed. In some examples, the system includes a junction disposed between a pump and a sample probe. In some examples, the pump is a peristaltic pump. A pulse-attenuation system is also disclosed. A method for removing gaps caused by pulsations in output data is also disclosed. A fluidics system and method for processing samples in parallel is also disclosed. A fluidic system for a flow cytometer that includes an agitator for agitating a sample is also disclosed. A sheath fluid transfer system of a fluidic system for a flow cytometer is also disclosed.
1. A flow cytometer comprising:
a flow cell for passing particles in a sample;
a sample probe to aspirate the sample;
a switching valve connected to the flow cell, the sample probe, a first tube section, and a second tube section, the switching valve having a first and a second selectable position, wherein the switching valve forms a fluid connection between the sample probe and the first tube section and between the flow cell and the second tube section when the switching valve is in the first position, and wherein the switching valve forms a fluid connection between the sample probe and the second tube section and between the flow cell and the first tube section when the switching valve is in the second position; and
a first pressure source connected to the first tube section, a second pressure source connected to the second tube section, and a third pressure source in fluid communication with a system fluid source, the system fluid source configured to provide system fluid,
wherein the first pressure source is configured to cause a first sample to flow from the sample probe to the first tube section, the second pressure source is configured to cause a second sample to flow from the second tube section to the flow cell when the switching valve is in the first position, and the third pressure source is configured to cause the system fluid to flow from the system fluid source, through the first tube section, and to the sample probe when the switching valve is in the first position.
2. The flow cytometer of claim 1 , wherein the switching valve comprises a rotary valve.
3. The flow cytometer of claim 1 , wherein the first pressure source is further configured to cause the first sample to flow from the first tube section to the flow cell when the switching valve is in the second position.
4. The flow cytometer of claim 3 , wherein the second pressure source is further configured to cause a third sample to flow from the sample probe to the second tube section when the switching valve is in the second position.
5. The flow cytometer of claim 1 , further comprising a flush valve disposed between the system fluid source and the first tube section to control the flow of system fluid from the first tube section to the sample probe.
6. The flow cytometer of claim 5 , wherein the flush valve is configured to prevent the flow of system fluid from the first tube section to the sample probe when the first pressure source causes sample to flow from the sample probe to the first tube section.
7. The flow cytometer of claim 1 , wherein the third pressure source is further configured to cause the system fluid to flow from the system fluid source, through the second tube section, and to the sample probe when the switching valve is in the second position.
8. The flow cytometer of claim 7 , further comprising a flush valve disposed between the system fluid source and the second tube section to control the flow of system fluid from the second tube section to the sample probe.
9. The flow cytometer of claim 8 , wherein the flush valve is configured to prevent the flow of system fluid from the second tube section to the sample probe when the second pressure source causes the sample to flow from the sample probe to the second tube section.
10. The flow cytometer of claim 8 , further comprising a flush valve disposed between the system fluid source and the first tube section to control the flow of system fluid from the first tube section to the sample probe.
11. The flow cytometer of claim 1 , further comprising a washing member in fluid communication with the system fluid source, the washing member being configured to wash an exterior surface of the sample probe.
12. The flow cytometer of claim 11 , wherein the washing member is a wash collar.
13. The flow cytometer of claim 11 , wherein the third pressure source is further configured to cause the system fluid to wash the exterior surface of the sample probe while the system fluid flows from the first tube section to the sample probe.
14. The flow cytometer of claim 1 , wherein the first pressure source is a syringe.
15. The flow cytometer of claim 1 , wherein the second pressure source is a syringe.
16. The flow cytometer of claim 1 , wherein the switching valve is a single valve having four ports.
17. A flow cytometer comprising:
a flow cell for passing particles in a sample;
a sample probe to aspirate the sample;
a switching valve connected to the flow cell, the sample probe, a first tube section, and a second tube section, the switching valve having a first and a second selectable position, wherein the switching valve comprises a pair of three-way valves and forms a fluid connection between the sample probe and the first tube section and between the flow cell and the second tube section when the switching valve is in the first position, and wherein the switching valve forms a fluid connection between the sample probe and the second tube section and between the flow cell and the first tube section when the switching valve is in the second position; and
a first pressure source connected to the first tube section, and a second pressure source connected to the second tube section,
wherein the first pressure source is configured to cause a first sample to flow from the sample probe to the first tube section and the second pressure source is configured to cause a second sample to flow from the second tube section to the flow cell when the switching valve is in the first position.
18. The flow cytometer of claim 17 , wherein each of the pair of three-way valves has three ports.
19. A flow cytometer comprising:
a flow cell for passing particles in a sample;
a sample probe to aspirate the sample;
a switching valve connected to the flow cell, the sample probe, a first tube section, and a second tube section, the switching valve having a first and a second selectable position, wherein the switching valve forms a fluid connection between the sample probe and the first tube section and between the flow cell and the second tube section when the switching valve is in the first position, and wherein the switching valve comprises four two-way valves, each two-way valve having two ports, and forms a fluid connection between the sample probe and the second tube section and between the flow cell and the first tube section when the switching valve is in the second position; and
a first pressure source connected to the first tube section, and a second pressure source connected to the second tube section,
wherein the first pressure source is configured to cause a first sample to flow from the sample probe to the first tube section and the second pressure source is configured to cause a second sample to flow from the second tube section to the flow cell when the switching valve is in the first position.