Increasing plant growth and yield by using an ERF transcription factor sequence
Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding ERF transcription factor proteins, polypeptides encompassing ERF transcription factor proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
1. A method for increasing seed yield in a plant, the method comprising:
(i) transforming a plant with a DNA construct comprising a recombinant polynucleotide which comprises a nucleotide sequence encoding an ERF (ethylene response factor) transcription factor protein having 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 2, wherein said nucleotide sequence is operably linked to a promoter that is functional in a plant cell; and
(ii) expressing said ERF protein in said transformed plant of step (i), and wherein said transformed plant from step (ii) that expresses said ERF protein exhibits increased seed yield as compared to a control plant of the same species lacking said DNA construct.
2. The method of claim 1 , wherein said ERF transcription factor protein has the amino acid sequence as set forth in SEQ ID NO: 2.
3. The method of claim 1 , wherein said promoter is SEQ ID NO: 5 or SEQ ID NO: 8.
4. The method of claim 1 , wherein said plant is a monocot.
5. The method of claim 1 , wherein said plant is a dicot.
6. The method of claim 1 , wherein said nucleotide sequence is set forth in SEQ ID NO: 1.