Mixtures of HMOs
The invention relates to a mixture of human milk oligosaccharides that consists essentially of: a) a component A which is 3-FL or DFL, a component B which is LNT, LNnT, LNFP-I or 2′-FL, a component C, which is LNFP-II when component B is LNT, or LNFP-III when component B is LNnT, or LNDFH-I when component B is LNFP-I, or DFL when component B is 2′-FL, and a component D, which is lactose when component A is 3-FL, or 2′-FL when component A is DFL, with the proviso that if component B is 2′-FL, then component A is 3-FL; or consists essentially of: b) 3-FL, a component E which is LNT, LNnT or LNFP-I, and a component F, which is LNFP-II when component E is LNT, or LNFP-III when component E is LNnT, or LNDFH-I when component E is LNFP-I, and to processes for producing them and their uses.
1. A process for obtaining the mixture of human milk oligosaccharides (HMOs) consisting essentially of components A, B, C, and D:
wherein
the component A is 3-fucosyllactose or difucosyllactose;
the component B is selected from the group consisting of lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose I, and 2′-fucosyllactose;
the component C is
lacto-N-fucopentaose II when the component B is lacto-N-tetraose,
lacto-N-fucopentaose III when the component B is lacto-N-neotetraose,
lacto-N-difucohexaose I when the component B is lacto-N-fucopentaose I, or
difucosyllactose when the component B is 2′-fucosyllactose; and
the component D is
lactose when the component A is 3-fucosyllactose, or
2′-fucosyllactose when the component A is difucosyllactose,
wherein the component A is 3-fucosyllactose when the component B is 2′-fucosyllactose; wherein the process comprises reacting the component A and the component B in the presence of an α1-3/4 transfucosidase to produce a reaction mixture, and then removing the α1-3/4 transfucosidase from the reaction mixture; and
wherein either
the component A is difucosyllactose and the process comprises reacting difucosyllactose and the component B in a molar ratio of 0.2-5 in the presence of an α1-3/4 transfucosidase, wherein the reaction has a conversion rate of at least 10%; or
the component A is 3-fucosyllactose, and the process comprises reacting 3-fucosyllactose and the component B in a molar ratio of 0.2-5 in the presence of an α1-3/4 transfucosidase, wherein the reaction has a conversion rate of at least 35%.
2. The process according to claim 1 , wherein the process is used to obtain the mixture wherein the component B is lacto-N-tetraose or lacto-N-neotetraose, and the molar ratio of (a) 3-fucosyllactose to the component C or (b) the component B to the component C is not more than 2.
3. The process according to claim 1 , wherein the process comprises reacting 3-fucosyllactose and the component B in a molar ratio of 0.33-3 in the presence of an α1-3/4 transfucosidase.
4. A process for obtaining a mixture of human milk oligosaccharides (HMOs) consisting essentially of:
3-fucosyllactose;
a component B selected from the group consisting of lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose I and 2′-fucosyllactose; and
a component C which is
lacto-N-fucopentaose II when the component B is lacto-N-tetraose,
lacto-N-fucopentaose III when the component B is lacto-N-neotetraose,
lacto-N-difucohexaose I when the component B lacto-N-fucopentaose I, or
difucosyllactose when the component B is 2′-fucosyllactose; and
comprising reacting 3-fucosyllactose and the component B in the presence of an α1-3/4 transfucosidase to produce a reaction mixture, and then removing lactose and the α1-3/4 transfucosidase from the reaction mixture;
wherein the process comprises reacting 3-fucosyllactose and the component B in a molar ratio of 0.2-5 in the presence of an α1-3/4 transfucosidase, wherein the reaction has a conversion rate of at least 35%.
5. The process according to claim 4 , wherein the mixture of HMOs consists essentially of:
3-fucosyllactose;
a component B selected from the group consisting of lacto-N-tetraose, lacto-N-neotetraose, and lacto-N-fucopentaose I; and a component C which is
lacto-N-fucopentaose II when the component E is lacto-N-tetraose,
lacto-N-fucopentaose III when the component E is lacto-N-neotetraose, or
lacto-N-difucohexaose I when the component E is lacto-N-fucopentaose I.