Method for screening activator of mitochondrial activity
The present invention relates to a method for screening an activator of mitochondrial activity by using gypenoside-treated cells, to a composition for screening an activator of mitochondrial activity, comprising the gypenoside, and to a kit comprising the composition. By using the method for screening an activator of mitochondrial activity of the present invention, it is possible to effectively discover a preparation which can substantially promote the mitochondrial activity, and thus the method is expected to be widely used in developing a therapeutic agent for diseases caused by mitochondrial activity inhibition.
1. A method for treating cells with an activator of mitochondrial activity comprising:
(a) isolating two cell groups containing the same cell type(s);
(b) treating a first group of cells from step (a) with a gypenoside, and treating a second group of cells from step (a) with candidate materials expected to promote mitochondrial activity, respectively; and
(c) measuring ATP production, intracellular mitochondrial membrane potential, and/or an amount of mitochondrial DNA for the first group of cells and the second group of cells.
2. The method of claim 1 , wherein the isolated cell groups are insulin-secreting cells.
3. The method of claim 1 , wherein the gypenoside is any one selected from the group consisting of gypenoside 1, gypenoside 3, gypenoside 4, gypenoside 5, gypenoside 8, gypenoside 17, gypenoside 48, gypenoside 75, and a combination thereof.
4. The method of claim 1 , wherein the gypenoside is gypenoside 75 Formula 1 below: