Compositions and methods for diagnosing, preventing and treating
The invention provides compositions and methods for preventing, treating and diagnosing infection by Salmonella enterica serovar typhi ( S. typhi ) and/or S. paratyphi , i.e., typhoid fever.
1. An immunological composition comprising
a.) a mutant Pertussin-like toxin A (PltA) polypeptide comprising the mutation E133A within SEQ ID NO: 8,
b.) a mutant Cytolethal distending toxin B (CdtB) polypeptide comprising the mutation H160Q within SEQ ID NO: 7 and
c.) a Pertussin-like toxin B (PltB) polypeptide.
2. A method of inducing an immune response in a subject, the method comprising administering a therapeutically effective amount of the immunological composition of claim 1 to the subject.
3. The method of claim 2 , wherein the polypeptide comprises at least one additional mutation that disrupts its enzymatic activity.
4. The method of claim 2 , wherein the immunological composition comprises a bacterium.
5. The method of claim 2 , further comprising administering an antibiotic to the subject.
6. The immunological composition of claim 1 wherein the-PltB polypeptide comprises a mutant PltB polypeptide comprising the mutation S35A within SEQ ID NO: 9.