Acidophilic fusarium oxysporum strains, methods of their production and methods of their use
The present invention provides isolated acidophilic Fusarium oxysporum strains, such as MK7, and their progeny, compositions comprising such strains and their progeny, methods of producing such strains and their progeny, and methods of using such strains and their progeny.
1. A method of producing one or more energy-rich metabolites, wherein the energy-rich metabolites are alcohols and/or hydrogen gas, said method comprising the steps of:
a) making a mixture by contacting an isolated Fusarium oxysporum strain MK7 (ATCC Accession Deposit No. PTA-10698) with a feedstock material selected from the group consisting of a lignocellulosic feedstock, a carbon containing waste product, a carbohydrate, and a combination thereof, wherein the feedstock material can support the growth of said Fusarium oxysporum strain MK7;
b) incubating said mixture at pH less than 5 for a period of time under anaerobic conditions;
c) producing from said mixture one or more energy-rich metabolites following such contact; and
d) optionally, isolating said one or more energy-rich metabolites from the mixture.
2. The method of claim 1 , wherein the lignocellulosic feedstock material is wheat straw.
3. The method of claim 1 , wherein the energy-rich metabolite is ethanol.
4. The method of claim 1 , wherein the feedstock is a lignocellulosic feedstock and the energy-rich metabolite is hydrogen gas.
5. The method of claim 1 , further comprising a pretreatment step prior to step (b), wherein said pretreatment step is selected from the group consisting of: reducing the pH of the mixture, adding manganese to the mixture and adding a nutrient to the mixture.
6. The method according to claim 5 , wherein the pH is 0.5-3.0 and the manganese concentration is 5 mM, 10 mM, or 25 mM.
7. The method according to claim 5 , wherein the pretreatment step is adding manganese to the mixture.
8. The method according to claim 7 , wherein the final manganese concentration in the mixture is 5 mM, 10 mM, or 25 mM.
9. The method according to claim 7 , wherein the manganese is selected from the group consisting of Mn 2 (CO) 10 , K 5 [Mn(CN) 6 NO, MnCl 2 , MnF 2 , MnBr 2 , MnO, MnO 2 , MnCl 3 , MnF 3 , MnBr 3 , MnCO 3 , Mn(CH 3 COO) 2 , C 6 H 9 MnO 6 , MnTiO 3 , [CH 3 COCH═C(O)CH 3 ] 2 Mn, [C 6 H 11 (CH 2 ) 3 CO 2 ] 2 Mn, (HCO 2 ) 2 Mn, Mn(C 5 HF 6 O 2 ) 2 , Mn(PH 2 O 2 ) 2 , MnI 2 , (C 3 H 5 O 3 ) 2 Mn, MnMoO 4 , Mn(NO 3 ) 2 , Mn(ClO 4 ) 2 , C 32 H 16 MnN 8 , MnSO 4 , (CH 3 COO) 3 Mn, C 32 H 16 ClMnN 8 , C 48 H 28 ClMnN 4 O 8 , C 5 H 4 CH 3 Mn(CO) 3 , Mn(C 5 H 4 C 2 H 5 ) 2 , and C 16 H 22 Mn.
10. The method according to claim 1 , wherein the pH is 0.5-3.0.
11. The method according to claim 1 , wherein the pH is 3-4.5.
12. The method according to claim 1 , wherein the energy-rich metabolite is a biofuel.
13. The method according to claim 1 , wherein the energy-rich metabolite is a biofuel precursor selected from the group consisting of isobutanol, isopropanol, propanol, 2-butanol, 30 butanol, pentanol, 2-pentanol, 3-pentanol, 3-methyl-1-butanol, 2-methyl-1-butanol, and 3-methyl-2-butanol.
14. The method according to claim 1 , wherein the carbon containing waste product is municipal, industrial, and/or farm sewage sludge.
15. The method according to claim 1 , wherein the carbohydrate is glucose.
16. The method according to claim 1 , wherein the energy-rich metabolite is butanol or isobutanol.