Method for producing an ActRIIB-Fc fusion polypeptide
In certain aspects, the present invention provides compositions and methods for modulating (promoting or inhibiting) growth of a tissue, such as bone, cartilage, muscle, fat, brown fat and/or neuronal tissue and for treating metabolic disorders such as diabetes and obesity, as well as disorders associated with any of the foregoing tissue.
1. A method for producing an ActRIIB-Fc fusion polypeptide, comprising:
a) culturing a host cell under conditions suitable for expression of the polypeptide, and
b) recovering the polypeptide expressed by the cell;
wherein the cell comprises a nucleic acid, wherein the nucleic acid encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 8.
2. The method of claim 1 , wherein the nucleic acid comprises the nucleotide sequence of SEQ ID NO: 4.
3. The method of claim 1 , wherein the nucleic acid comprises the nucleotide sequence of SEQ ID NO: 6.
4. The method of claim 3 , wherein the mammalian cell is a CHO cell.
5. The method of claim 1 , wherein the amino terminus of the polypeptide has the sequence ETR.
6. The method of claim 1 , wherein the host cell is a mammalian cell.
7. The method of claim 1 , wherein the nucleic acid is operably linked to one or more regulatory sequences.